HPV VIS Detail Information

> This page shows VIS [5001422] detail information, including site information (chromosome, GRCh38 location, disease, sample, etc) and literature information.


Site Information
DVID 5001422
VISID TVIS20000068
Chromosome chr8
GRCh38 Location 127828029
Disease Cervical carcinoma  
Sample Tumor
Virus Reference Genome NC_001526.4
Target Gene PVT1     
Literature Information
PubMed PMID 26735580
Published year 2016
Journal Oncotarget
Title Comprehensive mapping of the human papillomavirus (HPV) DNA integration sites in cervical carcinomas by HPV capture technology.
Author Liu Y,Lu Z,Xu R,Ke Y
Evidence Integration of human papillomavirus (HPV) DNA into the host genome can be a driver mutation in cervical carcinoma. Identification of HPV integration at base resolution has been a longstanding technical challenge, largely due to sensitivity masking by HPV in episomes or concatenated forms. The aim was to enhance the understanding of the precise localization of HPV integration sites using an innovative strategy. Using HPV capture technology combined with next generation sequencing, HPV prevalence and the exact integration sites of the HPV DNA in 47 primary cervical cancer samples and 2 cell lines were investigated. A total of 117 unique HPV integration sites were identified, including HPV16 (n = 101), HPV18 (n = 7), and HPV58 (n = 9). We observed that the HPV16 integration sites were broadly located across the whole viral genome. In addition, either single or multiple integration events could occur frequently for HPV16, ranging from 1 to 19 per sample. The viral integration sites were distributed across almost all the chromosomes, except chromosome 22. All the cervical cancer cases harboring more than four HPV16 integration sites showed clinical diagnosis of stage III carcinoma. A significant enrichment of overlapping nucleotides shared between the human genome and HPV genome at integration breakpoints was observed, indicating that it may play an important role in the HPV integration process. The results expand on knowledge from previous findings on HPV16 and HPV18 integration sites and allow a better understanding of the molecular basis of the pathogenesis of cervical carcinoma.

Contents
Description
  • Site Information
Detail information of site [5001422]
  • Literature Information
The details of literature that this site is associated with.