Literature Information
PubMed PMID
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30688658
|
Published year
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2019 |
Journal
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The Journal of clinical investigation |
Title
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Intact HIV-1 proviruses accumulate at distinct chromosomal positions during prolonged antiretroviral therapy. |
Author
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Einkauf KB,Lee GQ,Gao C,Sharaf R,Sun X,Hua S,Chen SM,Jiang C,Lian X,Chowdhury FZ,Rosenberg ES,Chun TW,Li JZ,Yu XG,Lichterfeld M |
Evidence
|
Chromosomal integration of genome-intact HIV-1 sequences into the host genome creates a reservoir of virally infected cells that persists throughout life, necessitating indefinite antiretroviral suppression therapy. Here, we used matched integration site and proviral sequencing (MIP-Seq), an experimental approach involving multiple displacement amplification of individual proviral species, followed by near-full-length HIV-1 next-generation sequencing and corresponding chromosomal integration site analysis to selectively map the chromosomal positions of intact and defective proviruses in 3 HIV-1-infected individuals undergoing long-term antiretroviral therapy. In addition, intact HIV-1 proviruses were preferentially integrated in either relative proximity to or increased distance from active transcriptional start sites and to accessible chromatin regions.
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