HPV VIS Detail Information

> This page shows VIS [5003616] detail information, including site information (chromosome, GRCh38 location, disease, sample, etc) and literature information.

Site Information

DVID	5003616
VISID	TVIS20004856
Chromosome	chr2
GRCh38 Location	20812529
Disease	Cervical carcinoma
Sample	Cell line
Virus Reference Genome	Not given

Literature Information

PubMed PMID	18922896
Published year	2008
Journal	Cancer research
Title	Characterization of naturally occurring HPV16 integration sites isolated from cervical keratinocytes under noncompetitive conditions.
Author	Dall KL,Scarpini CG,Roberts I,Winder DM,Stanley MA,Muralidhar B,Herdman MT,Pett MR,Coleman N
Evidence	As the high-risk human papillomavirus (HPV) integrants seen in anogenital carcinomas represent the end-point of a clonal selection process, we used the W12 model to study the naturally occurring integration events that exist in HPV16-infected cervical keratinocytes before integrant selection. We found that integration can occur at any time during episome maintenance, providing biological support for epidemiologic observations that persistent HPV infection is a major risk factor in cervical carcinogenesis. Of 24 different integration sites isolated from a single nonclonal population of W12, 12 (50%) occurred within chromosome bands containing a common fragile site (CFS), similar to observations for selected integrants in vivo. We further observed that local DNA rearrangements occur frequently and rapidly after the integration event. The majority of integrants were in chromosome bands containing a cancer-associated coding gene or microRNA, indicating that integration occurs commonly in these regions, regardless of selective pressure. The cancer-associated genes were generally a considerable distance from the integration site, and there was no evidence for altered expression of nine strong candidate genes. These latter observations do not support an important role for HPV16 integration in causing insertional mutagenesis.