HPV VIS Detail Information

> This page shows VIS [5000901] detail information, including site information (chromosome, GRCh38 location, disease, sample, etc) and literature information.

Site Information

DVID	5000901
VISID	TVIS20004424
Chromosome	chr3
GRCh38 Location	189849531
Disease	Oropharyngeal squamous cell carcinoma
Sample	Tumor
Virus Reference Genome	NC_001526.1
Target Gene	TP63

Literature Information

PubMed PMID	24586376
Published year	2014
Journal	PloS one
Title	Comprehensive analysis of HPV16 integration in OSCC reveals no significant impact of physical status on viral oncogene and virally disrupted human gene expression.
Author	Olthof NC,Speel EJ,Kolligs J,Haesevoets A,Henfling M,Ramaekers FC,Preuss SF,Drebber U,Wieland U,Silling S,Lam WL,Vucic EA,Kremer B,Klussmann JP,Huebbers CU
Evidence	However, it is unclear whether viral integration is an essential hallmark in the carcinogenic process of OSCC and whether HPV integration correlates with the level of viral gene transcription and influences the expression of disrupted host genes. Viral integration was examined using DIPS- as well as APOT-PCR. We identified 37 HPV16-human fusion products indicating viral integration in 29 (39%) OSCC. When comparing OSCC with or without an integration-derived fusion product, we did not find significant differences in the mean RNA expression of viral genes E2, E6 and E7 or the viral copy numbers per cell, nor did the RNA expression of the HPV-disrupted genes differ from either group of OSCC. In conclusion, our data do not support the hypothesis that integration affects the levels of viral and/or HPV-disrupted human gene transcripts.