HIV VIS Detail Information

> This page shows VIS [4006966] detail information, including site information (chromosome, GRCh38 location, disease, sample, etc) and literature information.

Site Information

Literature Information

PubMed PMID	30688658
Published year	2019
Journal	The Journal of clinical investigation
Title	Intact HIV-1 proviruses accumulate at distinct chromosomal positions during prolonged antiretroviral therapy.
Author	Einkauf KB,Lee GQ,Gao C,Sharaf R,Sun X,Hua S,Chen SM,Jiang C,Lian X,Chowdhury FZ,Rosenberg ES,Chun TW,Li JZ,Yu XG,Lichterfeld M
Evidence	Chromosomal integration of genome-intact HIV-1 sequences into the host genome creates a reservoir of virally infected cells that persists throughout life, necessitating indefinite antiretroviral suppression therapy. Here, we used matched integration site and proviral sequencing (MIP-Seq), an experimental approach involving multiple displacement amplification of individual proviral species, followed by near-full-length HIV-1 next-generation sequencing and corresponding chromosomal integration site analysis to selectively map the chromosomal positions of intact and defective proviruses in 3 HIV-1-infected individuals undergoing long-term antiretroviral therapy. In addition, intact HIV-1 proviruses were preferentially integrated in either relative proximity to or increased distance from active transcriptional start sites and to accessible chromatin regions.