Literature Information
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PubMed PMID
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26819646
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Published year
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2016 |
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Journal
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Journal of Cancer |
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Title
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Genome-wide Analysis of Epstein-Barr Virus (EBV) Integration and Strain in C666-1 and Raji Cells. |
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Author
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Xiao K,Yu Z,Li X,Li X,Tang K,Tu C,Qi P,Liao Q,Chen P,Zeng Z,Li G,Xiong W |
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Evidence
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EBV integration has been reported, but its scale and impact to cancer development is remains unclear. A rare few EBV integration sites in Raji were found in previous research by traditional methods. To deeply survey EBV integration, we sequenced C666-1 and Raji whole genomes by the next generation sequencing (NGS) technology and a total of 909 breakpoints were detected in the two cell lines. Moreover, we observed that the number of integration sites was positive correlated with the total amount of chromosome structural variations (SVs) and copy number structural variations (CNVs), and most breakpoints located inside or nearby genome structural variations regions. It suggested that host genome instability provided an opportunity for EBV integration on one hand and the integration aggravated host genome instability on the other hand. Thus, we report the most comprehensive characterization of EBV integration in NPC cell and BL cell, and EBV shows the wide range and random integration to increase the tumorigenesis. The NGS provides an incomparable level of resolution on EBV integration and a convenient approach to obtain viral strain compared to any research technology before.
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EBV VIS Detail Information