HBV VIS Detail Information

> This page shows VIS [1004845] detail information, including site information (chromosome, GRCh38 location, disease, sample, etc) and literature information.


Site Information
DVID 1004845
VISID TVIS10000010
Chromosome chr14
GRCh38 Location 56950918
Disease Hepatocellular carcinoma  
Sample Tumor
Virus Reference Genome JQ688405
Literature Information
PubMed PMID 30535432
Published year 2019
Journal Molecular medicine reports
Title Different types of viral‑host junction found in HBV integration breakpoints in HBV‑infected patients.
Author Ruan P,Dai X,Sun J,He C,Huang C,Zhou R,Cao Z,Ye L
Evidence The present study surveyed the characteristics of hepatitis B virus (HBV) integration in the liver genomes of patients with acute hepatitis B (AHB), carriers of inactive hepatitis B surface antigen (HBsAg), and patients with chronic hepatitis B (CHB) receiving antiviral treatment. 'Shortread' whole genome sequencing (WGS) with an average of 4,879x coverage for HBV integration was performed in three patients with AHB, two carriers of inactive HBsAg, and 13 patients with CHB receiving antiviral treatment. Conventional polymerase chain reaction and Sanger sequencing were used to verify integration breakpoints supported by at least two pairedend reads, and viralhost chimeric transcripts were surveyed simultaneously. HBV integration breakpoints were 100% identified with an average of 138. The numbers of HBV integration breakpoints were positively associated with the sequencing depth coverage numbers and levels of intrahepatic covalently closed circular DNA, respectively (P<0. Four types of viralhost junction in 14 HBV integration breakpoints were detected (two viral junctions mapped in the HBs gene, one in the Precore gene, and others within the HBx gene): Forward simple junction, reverse simple junction, forward and reverse complicated junction, and microhomology were found in many of the junctions. As a result, HBV can integrate into the host gene in the same manner as nonhomologous end joining and microhomologymediated end joining with numerous sites, and a close association may exist between HBV integration and patient prognosis. HBx integration may be indispensable for viralhost chimeric transcription and HBsAg may be produced from integrated DNA.

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