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Virus Dataset Sample Info

> Dataset: 30271481 Search Result


Summary
Item Summary
Project 30271481
Virus Name HBV
Sample Number 67
Disease Hepatocellular carcinoma (HCC)
Country China

Sample
ID Sample ID Age Gender Origin Detail
1 647 53 M China View
2 654 60 M China View
3 678 54 M China View
4 687 26 M China View
5 690 66 M China View
6 693 48 M China View
7 699 46 F China View
8 700 56 M China View
9 703 49 F China View
10 712 62 M China View
11 715 48 M China View
12 722 41 F China View
13 724 51 F China View
14 725 48 M China View
15 729 58 M China View
16 734 48 M China View
17 745 36 M China View
18 747 65 M China View
19 760 46 M China View
20 764 61 M China View
21 765 69 M China View
22 766 65 M China View
23 768 64 M China View
24 775 58 M China View
25 777 55 M China View
26 778 51 M China View
27 780 57 M China View
28 781 63 M China View
29 783 56 M China View
30 791 46 M China View
31 794 63 M China View
32 795 59 M China View
33 801 55 F China View
34 805 74 M China View
35 807 41 F China View
36 809 44 M China View
37 810 58 F China View
38 812 50 F China View
39 813 55 M China View
40 815 59 M China View
41 821 56 M China View
42 822 67 M China View
43 828 54 M China View
44 829 42 M China View
45 831 41 M China View
46 846 44 M China View
47 850 43 F China View
48 872 44 M China View
49 878 54 M China View
50 B3 32 M China View
51 B4 22 M China View
52 B5 34 M China View
53 SD086 21 M China View
54 SD077 30 M China View
55 SD083 43 M China View
56 SD090 41 M China View
57 SD079 28 M China View
58 B2 35 M China View
59 B6 40 M China View
60 B7 22 F China View
61 B8 27 M China View
62 B9 37 M China View
63 SD073 41 M China View
64 SD075 34 M China View
65 SD081 23 M China View
66 SD084 37 M China View
67 SD088 32 M China View

Literature
Item Summary
PMID 30271481
Title Molecular Characterization of HBV DNA Integration in Patients With Hepatitis and Hepatocellular Carcinoma
Abstract Infection by chronic hepatitis B virus (HBV) is one of the major causes of liver cirrhosis and primary hepatocellular carcinoma (HCC). Viral DNA integration into the host cell genome is a key mechanism of hepatocarcinogenesis. However, the molecular characterization and the potential clinical implications of HBV DNA integration into patients suffering from different hepatitis and HCC remain unclear. In this study, we analyzed HBV integrations in patients with hepatitis B and HCC using HBV probe-based capturing and next-generation sequencing. The results revealed that the sizes of the HBV integrations ranged from 28 bp to 3215 bp, including the full-length HBV DNA sequence. The integration breakpoints were preferentially distributed in the viral enhancer, X protein, and core protein regions of the HBV genome. The number of HBV integrations followed an increasing trend from hepatitis to HCC, which was positively correlated with the HBV virus load in patients with hepatitis. The number of HBV integrations in the HBeAg positive chronic hepatitis B group was significantly greater than that in the other hepatitis B groups (P < 0.05). However, the relative abundance of HBV integrations was significantly higher in HCC tissues than in the adjacent liver tissues. Interestingly, 61.6% (8/13) of HBV-human DNA integration fragments could be detected at the RNA level. Our results also showed that HBV integration-targeted genes (ITGs) were significantly enriched in many cancer-related pathways, such as MAPK, extracellular matrix (ECM)-receptor interaction, and the hedgehog signaling pathway. Individuals with HBV integrations exhibited shorter disease-free survival (DFS) and overall survival (OS) than those without HBV integrations in some ITGs including LINC00293 (long intergenic non-protein coding RNA 293; DFS P = 0.008, OS P = 0.009),_FSHB_(follicle stimulating hormone beta subunit; DFS P = 0.05, OS P = 0.186), and_LPHN3_(latrophilin-3; DFS P = 0.493, OS P = 0.033). This study determined the underlying mechanism of HBV DNA integration in liver diseases and laid the foundation for future studies on the pathogenesis of liver cancer.