Virus Dataset Sample Info

> Dataset: 25901726 Search Result

Summary

Item	Summary
Project	25901726
Virus Name	HBV
Sample Number	50
Disease	Hepatocellular carcinoma (HCC)
Country	China
Data Link	https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE65484 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE65485 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE65486

Sample

ID	Sample ID	Age	Gender	Origin	Detail
1	304T	79	M	China	View
2	305T	33	M	China	View
3	307T	57	F	China	View
4	308T	71	M	China	View
5	309T	40	M	China	View
6	310T	74	M	China	View
7	311T	56	M	China	View
8	312T	32	F	China	View
9	314T	31	M	China	View
10	315T	52	M	China	View
11	316T	44	M	China	View
12	317T	43	M	China	View
13	319T	51	F	China	View
14	320T	46	M	China	View
15	321T	41	M	China	View
16	322T	39	M	China	View
17	323T	56	M	China	View
18	325T	55	F	China	View
19	326T	51	M	China	View
20	327T	43	M	China	View
21	328T	43	M	China	View
22	329T	53	M	China	View
23	330T	31	M	China	View
24	332T	56	M	China	View
25	333T	66	M	China	View
26	334T	55	M	China	View
27	335T	49	M	China	View
28	336T	49	M	China	View
29	337T	41	M	China	View
30	339T	52	M	China	View
31	342T	65	M	China	View
32	345T	43	M	China	View
33	346T	54	M	China	View
34	347T	41	M	China	View
35	348T	45	M	China	View
36	349T	39	M	China	View
37	350T	74	M	China	View
38	351T	39	M	China	View
39	352T	62	M	China	View
40	353T	60	M	China	View
41	354T	52	M	China	View
42	355T	56	M	China	View
43	356T	71	F	China	View
44	357T	23	M	China	View
45	358T	55	F	China	View
46	359T	67	F	China	View
47	360T	0	M	China	View
48	361T	71	M	China	View
49	362T	36	M	China	View
50	363T	44	M	China	View

Literature

Item	Summary
PMID	25901726
Title	Identification of HBV-MLL4 Integration and Its Molecular Basis in Chinese Hepatocellular Carcinoma
Abstract	To gain molecular insights of HBV integration that may contribute to HCC tumorigenesis, we performed whole transcriptome sequencing and whole genome copy number profiling of hepatocellular carcinoma (HCC) samples from 50 Chinese patients. We identified a total of 33 HBV-human integration sites in 16 of 44 HBV-positive HCC tissues, which were enriched in HBV genotype C-infected patients. In addition, significantly recurrent HBV-MLL4 integration (18%; 8/44) was found in this cohort of patients. Using long-range PCR and Sanger sequencing, we comprehensively characterized gDNA and cDNA sequences that encode for the HBV-MLL4 transcripts, and we revealed that HBV integration into MLL4 exons led to much higher mRNA expression of MLL4 than the integration into MLL4 introns due to an alternative splicing mechanism. Moreover, the HBV-MLL4 integration occurred almost exclusively in CTNNB1 and TP53 wild-type patients. The integration was also associated with a distinct gene expression profile. In conclusion, this is the first report on the molecular basis of the MLL4 integration driving MLL4 over-expression. HBV-MLL4 integration occurred frequently in Chinese HCC patients, representing a unique molecular segment for HCC with HBV infection.