Virus Dataset Sample Info

> Dataset: 22947305 Search Result


Summary
Item Summary
Project 22947305
Virus Name HTLV1
Sample Number 24
Disease HIV-HTLV-1 co-infection
Country Brazil
Data Link https://www.ncbi.nlm.nih.gov/nuccore/?term=JF271843:JF271852[pacc]
https://www.ncbi.nlm.nih.gov/nuccore/?term=JX280947:JX280960[pacc]

Sample
ID Sample ID Age Gender Origin Detail
1 BRSP134-08 45 M Brazil View
2 BRSP145-08 50 M Brazil View
3 BRSP206-08 42 F Brazil View
4 BRSP232-08 56 F Brazil View
5 BRSP42-09 55 F Brazil View
6 BRSP205-09 27 F Brazil View
7 BRSP320-09 51 F Brazil View
8 BRSP414-09 62 M Brazil View
9 BRSP425-09 57 F Brazil View
10 BRSP01-10 32 F Brazil View
11 BRSP30-10 38 F Brazil View
12 BRSP37-10 43 M Brazil View
13 BRSP38-10 45 M Brazil View
14 BRSP71-10 33 M Brazil View
15 BRSP93-10 43 F Brazil View
16 BRSP97-10 37 M Brazil View
17 BRSP101-10 40 F Brazil View
18 BRSP105-10 41 M Brazil View
19 BRSP118-10 65 M Brazil View
20 BRSP171-10 30 F Brazil View
21 BRSP40-11 46 M Brazil View
22 BRSP142-11 57 F Brazil View
23 BRSP194-11 37 F Brazil View
24 BRSP69-12 50 F Brazil View

Literature
Item Summary
PMID 22947305
Title LTR point mutations in the Tax-responsive elements of HTLV-1 isolates from HIV/HTLV-1-coinfected patients.
Abstract BACKGROUND: In Virology Journal 2011, 8:535, Neto et al. described point mutations into Tax-responsive elements (TRE) of the LTR region of HTLV-1 isolates from asymptomatic carriers from Sao Paulo, Brazil, and hypothesized that the presence of the G232A mutation in the TRE-1 increase viral proliferation and consequently the proviral load (PvL), while the A184G mutation in the TRE-2 do not have such effect. FINDINGS: We performed the real-time PCR assay (pol) and sequenced LTR region of HTLV-1 isolates from 24 HIV/HTLV-1-coinfected patients without HTLV-1-associated diseases from the same geographic area. These sequences were classified as belonging to the transcontinental subgroup A of the Cosmopolitan subtype a. The frequency of G232A mutation (16/24, 66.7%) was high as much as 61.8% reported by Neto's in HTLV-1 asymptomatic carriers with high PvL. High frequency (13/24, 54.2%) of double mutations G232A and A184G was also detected in HIV/HTLV-1-coinfected patients. We did not quantify PvL, but comparative analyses of the cycle threshold (Ct) median values of the group of isolates presenting the mutated-types sequences (Ct 33.5, n = 16) versus the group of isolates with the wild-type sequences (Ct 32, n = 8) showed no statistical difference (p = 0.4220). CONCLUSION: The frequencies of mutated-type sequences in the TRE-1 and TRE-2 motifs were high in HIV/HTLV-1-coinfected patients from Sao Paulo, Brazil. If these LTR point mutations have predictive value for the development of HTLV-1-associated diseases or they correspond to the subtype of virus that circulate in this geographic area has to be determined.