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> Dataset: 20865165 Search Result


Summary
Item Summary
Project 20865165
Virus Name MCV
Sample Number 33
Disease Merkel cell carcinoma (MCC)
Country India

Sample
ID Sample ID Age Gender Origin Detail
1 1 74 F India View
2 2(e) 81 M India View
3 3(e) 78 M India View
4 4 73 F India View
5 5 61 F India View
6 6 88 M India View
7 7(e) 84 M India View
8 8 67 F India View
9 9(f) 82 M India View
10 10 82 F India View
11 11 77 F India View
12 12 55 M India View
13 13 71 M India View
14 14(e,f) 63 M India View
15 15 86 F India View
16 16(f) 77 F India View
17 17 65 F India View
18 18 68 M India View
19 19 66 F India View
20 20 85 F India View
21 21 60 M India View
22 22 81 F India View
23 23(e) 85 M India View
24 24 71 F India View
25 25 63 F India View
26 26 39 F India View
27 27 81 F India View
28 28 58 F India View
29 29 77 F India View
30 30 80 M India View
31 31(e,f) 84 M India View
32 32 60 M India View
33 33 81 F India View

Literature
Item Summary
PMID 20865165
Title Distinct Merkel Cell Polyomavirus Molecular Features in Tumour and Non Tumour Specimens From Patients With Merkel Cell Carcinoma
Abstract Merkel Cell Polyomavirus (MCPyV) is associated with Merkel Cell carcinoma (MCC), a rare, aggressive skin cancer with neuroendocrine features. The causal role of MCPyV is highly suggested by monoclonal integration of its genome and expression of the viral large T (LT) antigen in MCC cells. We investigated and characterized MCPyV molecular features in MCC, respiratory, urine and blood samples from 33 patients by quantitative PCR, sequencing and detection of integrated viral DNA. We examined associations between either MCPyV viral load in primary MCC or MCPyV DNAemia and survival. Results were interpreted with respect to the viral molecular signature in each compartment. Patients with MCC containing more than 1 viral genome copy per cell had a longer period in complete remission than patients with less than 1 copy per cell (34 vs 10 months, P = 0.037). Peripheral blood mononuclear cells (PBMC) contained MCPyV more frequently in patients sampled with disease than in patients in complete remission (60% vs 11%, P = 0.00083). Moreover, the detection of MCPyV in at least one PBMC sample during follow-up was associated with a shorter overall survival (P = 0.003). Sequencing of viral DNA from MCC and non MCC samples characterized common single nucleotide polymorphisms defining 8 patient specific strains. However, specific molecular signatures truncating MCPyV LT were observed in 8/12 MCC cases but not in respiratory and urinary samples from 15 patients. New integration sites were identified in 4 MCC cases. Finally, mutated-integrated forms of MCPyV were detected in PBMC of two patients with disseminated MCC disease, indicating circulation of metastatic cells. We conclude that MCPyV molecular features in primary MCC tumour and PBMC may help to predict the course of the disease.