Virus Dataset Sample Info

> Dataset: 18320596 Search Result

Summary

Item	Summary
Project	18320596
Virus Name	HBV
Sample Number	32
Disease	Hepatocellular carcinoma (HCC)
Country	Japan

Sample

ID	Sample ID	Age	Gender	Origin	Detail
1	HCC131	48	M	Japan	View
2	HCC146	45	M	Japan	View
3	HCC002	58	M	Japan	View
4	HCC003	56	M	Japan	View
5	HCC9907	50	M	Japan	View
6	HCC155	52	M	Japan	View
7	H20	66	F	Japan	View
8	H54	29	F	Japan	View
9	H120	41	M	Japan	View
10	HCC143	63	M	Japan	View
11	H49	66	M	Japan	View
12	H62	62	M	Japan	View
13	H70	53	M	Japan	View
14	H72	62	M	Japan	View
15	H78	66	M	Japan	View
16	H89	61	F	Japan	View
17	H76	71	M	Japan	View
18	H57	66	M	Japan	View
19	H71	60	M	Japan	View
20	H85	55	M	Japan	View
21	H86	65	M	Japan	View
22	H87	62	M	Japan	View
23	HCC128	73	M	Japan	View
24	HCC147	68	M	Japan	View
25	HCC127	57	M	Japan	View
26	HCC001	68	M	Japan	View
27	H148	70	F	Japan	View
28	H149	66	M	Japan	View
29	H150	60	M	Japan	View
30	HCC9833	70	M	Japan	View
31	HCC9901	69	M	Japan	View
32	HCC9906	69	F	Japan	View

Literature

Item	Summary
PMID	18320596
Title	Integration of Hepatitis B Virus DNA Into the Myeloid/Lymphoid or Mixed-Lineage Leukemia (MLL4) Gene and Rearrangements of MLL4 in Human Hepatocellular Carcinoma
Abstract	Integration of hepatitis B virus (HBV) DNA into host DNA is detected in about 90% of HBV-related hepatocellular carcinoma (HCC), but the preferential sites of the viral integration etiologically relevant to oncogenesis have been controversial. By using an adaptor-ligation/suppression-PCR, we identified four integrations into the myeloid/lymphoid or mixed-lineage leukemia 4 (MLL4) gene from 10 HCC patients with positive HBV surface antigen (HBsAg). Determination of the cellular-virus DNA junction demonstrated that various lengths of the virus were integrated within 300 bp of intron 3 flanked by the Alu element of MLL4. Chimeric hepatitis B virus X gene (HBx)/MLL4 transcripts and the HBx fusion proteins were detected. DNA microarray revealed that HBx/MLL4 fusion proteins suppressed unique genes in HepG2 cells. Finally, chromosomal translocations of intron 3 of MLL4 to the specific region of chromosome 17p11.2 in 22 out of 32 HCC patients were observed, showing that the intron 3 region of MLL4 gene would be a target of translocation breakpoint. In conclusion, the present data suggest that the translocation breakpoint of MLL4 gene is one of the preferential targets for HBV DNA integration into the MLL4 gene and the HBV DNA integration may be involved in liver oncogenesis.