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Mutation Information
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Mutation Site
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M133T |
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Mutation Type
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Amino acid level |
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Gene/Protein/Region Type
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S |
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Genotype/Subtype
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D |
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Country
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US |
Literature Information
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PubMed PMID
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32512942
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Published Year
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2020 |
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Journal
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Cells |
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Title
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N-Glycosylation and N-Glycan Processing in HBV Biology and Pathogenesis. |
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Author
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Dobrica MO,Lazar C,Branza-Nichita N |
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Evidence
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The steps in the HBV life-cycle regulated by N-glycosylation/N-glycan processing are depicted by thick red arrows. Mutation Glycosylation Properties Reference T123N Yes Poor recognition by HBsAg antibodies (Abs)Triggers anti-HBsAg humoral responseStrongly reduces assembly of HBV (genotype C) Significantly reduced reactivity with commercial HBsAg ELISA kitsAble to induce protective HBsAg Abs titersNormal viral replication Isolated from patients with HBV infectionReduced reactivity with commercial HBsAg ELISA kitsHBsAg negativity at reactivationNo effect on HBV replication (genotype D)No effect on HBsAg secretion T115NT117N114N-insertionS113N+T131N+M133T * Yes Isolated from patients with HBV infectionReduced reactivity with commercial HBsAg ELISA kitsHBsAg negativity at reactivationNo effect on HBV replication (genotype D)No effect on HBsAg secretion K160N Yes Poor recognition by HBsAg AbsTriggers anti-HBsAg humoral responseEnhanced cellular immune responseCompensates for the negative effect of A159G mutation on HBV production G130N ** NE Weak recognition by a conformational HBsAg Abs Q129N Yes Decreased binding to HBsAg AbsIncreased HBV production in trans-complementary studies Impaired recognition by HBsAg AbsPoor immunogenicity, still protective HBsAg Abs titersImpairs viral replication but not HBsAg secretion Decreased binding to HBsAg Abs (by 70.3%)Increased HBV secretion in trans-complementary studies *** T131N/M133T # Yes Impaired recognition by HBsAg AbsPoor immunogenicity, still protective HBsAg Abs titersNormal viral replication Decreased binding to HBsAg Abs (by 84.5%)Increased HBV secretion in trans-complementary studies *** T131N # Yes Decreased binding to anti-HBsAg AbsIncreased HBV production in trans-complementary studies "RPCMNCTI" insertion between residues 126-127 Yes Moderate decrease of HBsAg Abs binding (by 23.2%)Normal HBV secretion in trans-complementary studies *** 3 aa insertion between residues 114-115 Yes Decreased binding to anti-HBsAg AbsIncreased HBV production in trans-complementary studies M133T # Yes Increased virion secretionRestores virion secretion of G119E, G145R, I110M, R169P immune-escape mutants but not recognition by the neutralizing Abs.
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HBV Mutation Detail Information