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Mutation Information
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Mutation Site
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F195A |
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Mutation Type
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Amino acid level |
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Gene/Protein/Region Type
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Nef |
Literature Information
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PubMed PMID
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33146906
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Published Year
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2020 |
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Journal
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The EMBO journal |
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Title
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HIV-1 infection of CD4 T cells impairs antigen-specific B cell function. |
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Author
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Kaw S,Ananth S,Tsopoulidis N,Morath K,Coban BM,Hohenberger R,Bulut OC,Klein F,Stolp B,Fackler OT |
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Evidence
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This included NEF F195A which lacks the ability to associate with the cellular p21‐activated kinase 2 (PAK2) to negatively modulate host cell actin dynamics and motility (O'Neill et al, 2006; Stolp et al, 2009; Stolp et al, 2012), a NEF variant with disrupted di‐leucine motif (NEF LLAA) that lacks the ability to internalize cell surface receptors such as CD4 (Craig et al, 1998; Greenberg et al, 1998), NEF AxxA in which an SH3 domain‐binding PxxP motif is disrupted and fails, e.g., to relocalize the TCR proximal kinase Lck from the plasma membrane to intracellular compartments (Saksela et al, 1995; Pan et al, 2012), as well as NEF variants carrying a deletion of an N‐terminal protein interaction platform (NEF ∆12‐39) or a mutation in an interaction motif within residues 12‐39 (NEF A32‐39) required for CD4 downregulation and SERINC5 antagonism (Ananth et al, 2019).
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HIV Mutation Detail Information