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Mutation Information
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Mutation Site
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D116N |
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Mutation Type
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Amino acid level |
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Gene/Protein/Region Type
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IN |
Literature Information
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PubMed PMID
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33571428
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Published Year
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2021 |
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Journal
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Cell |
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Title
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Cone-shaped HIV-1 capsids are transported through intact nuclear pores. |
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Author
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Zila V,Margiotta E,Turoňová B,Müller TG,Zimmerli CE,Mattei S,Allegretti M,Börner K,Rada J,Müller B,Lusic M,Kräusslich HG,Beck M |
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Evidence
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Plasmid pNNHIV (pNLC4-3 IND64N/D116N tatΔ33-64bp) for production of non-infectious, RT-competent HIV-1 particles, was constructed by successive introduction of integrase catalytic mutations D64N and D116N by Quickchange PCR into pUC19 NL4-35’CA-3’Vpr (generated by subcloning a 4296 bp SphI/EcoRI fragment from pNL4-3 into pUC19, from which the NdeI site has been removed by Klenow insertion and religation).
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HIV Mutation Detail Information