Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69-V70, France, August to December 2020.
Introduction: This cluster-5 variant carries a receptor binding domain (RBD) mutation Y453F and was associated with reduced susceptibility to neutralising antibodies of sera from recovered coronavirus disease (COVID-19) patients.
Discussion: It should be underlined that N439K, Y453F or N501Y RBD mutations that can co-occur with DeltaH69/DeltaV70 might be associated with an increased affinity to angiotensin-converting enzyme 2 (ACE2) or reduced sensitivity to SARS-CoV-2 antibodies.
Intranasal Infection of Ferrets with SARS-CoV-2 as a Model for Asymptomatic Human Infection.
Abstract: Viral genomic sequence analysis in samples from three animals identified the Y453F nucleotide substitution relative to the inoculum.
Discussion: Although the SARS-CoV-2 spike protein from human isolates is predicted to have low affinity for the mink and ferret ACE2 receptors, the emergence of the Y453F variant in both field and experimental infection of mink and ferrets may indicate that this mutation promotes a functional interaction between virus spike protein and mink or ferret ACE2 receptors.
Discussion: Another single nucleotide polymorphism corresponded to the spike protein Y453F variant that has emerged in mink in the Netherlands, as well as being present in the Cluster 5 variant of SARS-CoV-2 isolated from mink in Denmark and in isolated human clinical cases.|
Vaccine-escape and fast-growing mutations in the United Kingdom, the United States, Singapore, Spain, India, and other COVID-19-devastated countries.
Introduction: SARS-CoV-2 variants isolated from minks and mouse harboring mutations G261D, A262S, L452M, Y453F, F486L, Q498H and N501T may cause potential cross-species transmission that worth closely monitor (Thomson et al.; Yao et al.).
Insilico study on the effect of SARS-CoV-2 RBD hotspot mutants' interaction with ACE2 to understand the binding affinity and stability.
Result: The variants with more negative binding free energy (high binding affinity) than the WT complex are N501Y, N501T, K417R, N501I, L455F, A475V, N501S, Y453F, Q493H, G446S, G446V, Q493L and Y495F.
Table: Y453F
The emerging SARS-CoV-2 variants of concern.
PMID: 34211709
2021
Therapeutic advances in infectious disease
Abstract: These mutations carry a lineage from N501Y, D614G, N439K, Y453F, and others, which are globally dominated by clades 20A, 20B, and 20C.
Method: Cluster 5 or Y453F variant, which emerged in August of 2020, may potentially evade the immunity of convalescent individuals, further suggesting that this variant may challenge the vaccine strategy should it spread.
Method: They include but were not limited to COVID-19, SARS-CoV-2, Variants of Concern, genetic variations, spike mutations, N501Y, D614G, N439K, Y453F, E484K, B.1.1.7, 501Y.V1, 501Y.V2, 501.V3, a
Use of Lateral Flow Immunoassay to Characterize SARS-CoV-2 RBD-Specific Antibodies and Their Ability to React with the UK, SA and BR P.1 Variant RBDs.
Introduction: Figure 1 summarizes currently circulating SARS-CoV-2 variants and their respective mutations within the spike RBD, which include the following: N501Y in the UK, SA, and BR-P.1 variants; E484K/Q in the SA, BR P.1, BR P.2, NY, and IN variants; K417N/T in the SA and BR P.1 variants; L452R in the CA and IN variants; S477N in some NY variants; and Y453F in the Denmark mink variant.
Anti-SARS-CoV-2 Vaccines and Monoclonal Antibodies Facing Viral Variants.
Result: Specifically, N501Y and Y453F combined with N439K increased affinity for human ACE2 by 5-fold (Figures 4D and S3 ).
Result: Using biolayer interferometry (BLI), we measured association and dissociation rates of the N501Y RBD mutant (B.1.1.7 carries that mutation as its sole RBD mutation), Y453F, as found in mink isolates, N439K, which is found in some European clades, a combination of Y453F and N439K, E484K (part of B.1.351 and P.1) as well as for the B.1.351 and the P.1 RBDs for a recombinant version of human ACE2 (Figures 4A, 4B, and 4D).
Table:
SARS-CoV-2 spike L452R variant evades cellular immunity and increases infectivity.
Result: Additionally, the three sequences that contain the Y453F mutation were isolated from cats in Denmark: two identical sequences (GISAID ID: EPI_ISL_683164 and EPI_ISL_683166) and another sequence (GISAID ID: EPI_ISL_683165) of a distinct origin (Figure S2).
Result: Altogether, these results suggest that the NF9 peptide, which is derived from the SARS-CoV-2 S protein RBM, is an immunodominant epitope of HLA-A24 and that two naturally occurring mutants, L452R and Y453F, are able to evade HLA-A24-restricted cellular immunity.
Result: Consistent with recent studies, including ours, the N501Y mutation, which is a common mutation in B1.1.7, B1.351, and P.1 variants (reviewed in), as well as the Y453F mutation, significantly increased binding affinity for human ACE2 (Figures 2
SARS_CoV_2 mutation literature information.
PMID: 
2021
Euro surveillance
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