Introduction: Here, we describe three infections with rare E484K-harboring SARS-CoV-2 containing three other amino acid substitutions in the spike, including W152L, which is located in the N-terminal domain (NTD) and possibly reduces sensitivity to neutralizing antibodies, and
Introduction: However, the loss of affinity of the W152L mutant for gangliosides is not anecdotical.
Introduction: The electrostatic surface potential of the NTD was also increased by the mutation W152L, but by an indirect mechanism of compaction of the domain that decreases the electronegative areas in favor of an enlarged electropositive surface.
Figure: (C) Effect of the W152L mutation on the electrostatic surface potential of the NTD (upper panels).
Intra-host non-synonymous diversity at a neutralizing antibody epitope of SARS-CoV-2 spike protein N-terminal domain.
PMID: 33144203
2021
Clinical microbiology and infection
Abstract: DISCUSSION: A spike protein amino acid mutation W152L located within a neutralizing epitope has appeared naturally in a patient.
Result: Of 92942 sequences available, 21 (0.023%) had W152L mutation.
Discussion: Fourth, for the patient with W152L mutation in the saliva specimen collected on day 9 after symptom onset, only sputum specimen was available on day 7 after symptom onset.
Discussion: However, out of 4000 bp sequenced, only W152L was found to be the predominant mutant.
Discussion: However, since usually only one viral sequence from each patient would be deposited into public databases, the prevalence of W152L mutation may have been underestimated.
Discussion: In one patient with severe disease, an important non-synonymous mutation <
Detection of R.1 lineage severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with spike protein W152L/E484K/G769V mutations in Japan.
Figure: We analyzed samples of R.1 lineage SARS-CoV-2 (n = 3), which harbors a spike variant with the W152L/E484K/G769V mutations and samples of SARS-CoV-2 without these three mutations (n = 5) using a TaqMan assay.
Discussion: Furthermore, the W152L mutation is located in the N3 loop of the NTD and may be related to decreased antibody neutralization activity.
Discussion: In the present study, we observed an expansion in Japan of R.1 lineage SARS-CoV-2 harboring the spike RBD E484K and spike NTD W152L mutations, which have potential significance for immune escape.
Trajectory of Growth of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Variants in Houston, Texas, January through May 2021, Based on 12,476 Genome Sequences.
PMID: 34303698
2021
The American journal of pathology
Result: R.1 has the following core spike protein amino acid changes: W152L, E484K, D614G, and G769V (Outbreak.info, https://outbreak.info/situation-reports?pango=r.1, last accessed May 17, 2021).
Mutational analysis in international isolates and drug repurposing against SARS-CoV-2 spike protein: molecular docking and simulation approach.
Result: WGS analysis of the selected samples revealed that the 501N + 484K type is an R.1 lineage (W152L, E484K, D614G, G769V) variant, which is the sublineage of B.1.1.316.
Detection and characterization of the SARS-CoV-2 lineage B.1.526 in New York.
SARS-CoV-2 B.1.1.7 lineage rapidly spreads and replaces R.1 lineage in Japan: Serial and stationary observation in a community.
PMID: 34560289
2021
Infection, genetics and evolution
Introduction: We previously reported on the SARS-CoV-2 R.1 lineage harboring spike W152L, E484K and G769V mutations.
Discussion: The R.1 carries the W152L N-terminal domain and E484K in receptor binding domain, which have been shown to be of concern for immune escape, while there are no reports on its transmission potential.
Temporal-Geographical Dispersion of SARS-CoV-2 Spike Glycoprotein Variant Lineages and Their Functional Prediction Using in Silico Approach.
Result: Dual mutants containing D614G and other amino acid changes, including those under positive selection or observed in VOCs (L5F, L18F, S98F, W152L/C, E154K, L222V, and A262S in S1-NTD; N439K, L452Q/R, S477N, L478R/K, E484K/Q, N501Y, and A570D within S1-RBD; Q677H/P and P681H/R in S1-CTD; T716I,
A rigorous framework for detecting SARS-CoV-2 spike protein mutational ensemble from genomic and structural features.
PMID: 34806033
2021
Current research in structural biology
Result: Specifically, L18F, T19R, and T20N are present in the N-terminus epitope, whereas G142D, W152 R/L/C, M153T, and F157 are present in the supersite beta-hairpin epitope.
SARS_CoV_2 mutation literature information.
PMID: 
2022
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