literature

SARS_CoV_2 mutation literature information.

Characterizing SARS-CoV-2 mutations in the United States.

PMID: 32818213 2020 Research square

Result: Notably, the mutation 23010T>C-(V483A) has the highest frequency (30) localized on the RBM has the positive binding affinity change, which indicates that V483A is prevalent in COVID-19 patients' in the United States has a potential capacity to enhance the infectivity of SARS-CoV-2.

Result: The V483A mutation is localized on the RBM with the highest frequency, indicating that V483A may favor SARS-CoV-2 by natural selection and cause SARS-CoV-2 more infectious.

Variant analysis of SARS-CoV-2 genomes.

PMID: 32742035 2020 Bulletin of the World Health Organization

Result: Among the variants in the receptor binding domain, V483A (26 samples), G476S (9 samples) and V367F (12 samples) are highly recurrent.

Discussion: V483A and G476S are primarily observed in samples from the United States, whereas V367F is found in samples from China, Hong Kong Special Administrative Region, France and the Netherlands.

The Impact of Mutations in SARS-CoV-2 Spike on Viral Infectivity and Antigenicity.

PMID: 32730807 2020 Cell

Abstract: Most variants with amino acid change at receptor binding domain were less infectious, but variants including A475V, L452R, V483A, and F490L became resistant to some neutralizing antibodies.

Result: Moreover, V483A became resistant to mAbs X593 and P2B-2F6, and L452R to mAbs X593 and P2B-2F6.

Result: Neither X593 nor P2B-2F6 was effective in neutralizing L452R, V483A, and F490L, whereas P2B-2F6 was more effective in neutralizing N165Q.

Bivalent binding of a fully human IgG to the SARS-CoV-2 spike proteins reveals mechanisms of potent neutralization.

PMID: 32699850 2020 bioRxiv

Result: 5A6 IgG had a 4-fold reduction in binding avidity to the V483A mutation but was insensitive to the other five mutations.

Result: Consistent with the binding results, 5A6 significantly lost neutralizing capacity against the V483A mutant pseudovirus.

Result: Hence, 3D11 could potentially rescue the loss of 5A6 neutralization potency in a virus strain bearing the V483A mutation, if these two antibodies are used in combination.

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