literature

Recent progress on the mutations of SARS-CoV-2 spike protein and suggestions for prevention and controlling of the pandemic.

PMID: 34146731 2021 Infection, genetics and evolution

Introduction: Among the mutations in the B.1.1.7, nine mutations, including H69-V70 deletion (Delta69/Delta70), Y144 deletion (Delta144), N501Y, A570D, D614G, P681H, T716I, S982A, and D1119H, were located in the viral spike protein.

Introduction: The 501Y Variant 2 contains 17 mutations, including H69-V70 deletion (Delta69/Delta70), Y144 deletion (Delta144), N501Y, A570D, P681H, T716I, S982A, D1118H, T1001I, A1708D,

PMID: 34160124 2021 Chembiochem

Introduction: These mutations in this strain may enhance virus transmissibility and infectivity, including the deletion of residues 69-70 and 144 and the substitution of A570D, D614G, T716I, S982A, D1118H, P681H, K417N, K417T, E484 K and N501Y.

Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the Alpha variant B.1.1.7.

PMID: 34166617 2021 Cell reports

Method: The mutations (Delta69/70, Delta144, N501Y, A570D, D614G, P681H, S982A, T716I and D1118H or K417N, E484K and N501Y) were introduced by amplification with primers with similar Tm.

Result: In addition to RBD N501Y and NTD DeltaH69/V70, B.1.1.7 is defined by further S mutations across S2 (T716I, S982A, and D1118H) and S1 (DeltaY144, A570D, and

Analysis of SARS-CoV-2 variant mutations reveals neutralization escape mechanisms and the ability to use ACE2 receptors from additional species.

PMID: 34166623 2021 Immunity

Method: The variant B.1.1.7 (GISAID: EPI_ISL_601443) was constructed with total of 9 mutations including 69-70del, 144del, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H.

Emergence in southern France of a new SARS-CoV-2 variant harbouring both N501Y and E484K substitutions in the spike protein.

PMID: 34170525 2021 Journal of medical virology

Result: For each sample, a total of nine mutations were found in the spike protein: H69-70-, Y144-, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H.

Specific allelic discrimination of N501Y and other SARS-CoV-2 mutations by ddPCR detects B.1.1.7 lineage in Washington State.

PMID: 34170525 2021 Journal of medical virology

Discussion: The large number of genomic mutations associated with B.1.1.7, including SNPs (A570D, D614G, P681H, T716I, S982A, and D1118H) highlight the need to rapidly distinguish these subtle mutations in emerging VOCs.

Immune Evasion of SARS-CoV-2 Emerging Variants: What Have We Learnt So Far?

PMID: 34206453 2021 Viruses

Introduction: The first variant Alpha or B.1.1.7 that raised global concerns about increased transmissibility and potential immune evasion harbors seven missense mutations (N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) and three deletions in spike (69/70del and 144del) (Figure 3).

Anti-SARS-CoV-2 Vaccines and Monoclonal Antibodies Facing Viral Variants.

PMID: 34207378 2021 Viruses

Introduction: It is characterized by an insertion of three amino acids (Ins214TDR) and four mutations in the Spike gene: Q414K, N450K, D614G, and T716I.

A Comprehensive Molecular Epidemiological Analysis of SARS-CoV-2 Infection in Cyprus from April 2020 to January 2021: Evidence of a Highly Polyphyletic and Evolving Epidemic.

PMID: 34207490 2021 Viruses

Result: These mutations/deletions were DeltaH69/V70, S98F, DeltaY144, S162G, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H.

Discussion: The following mutations within the S protein have been reported in this lineage: N501Y, A570D, P681H, T716I, S982A, D1118H, DeltaH69/V70 and DeltaY144.

Insilico study on the effect of SARS-CoV-2 RBD hotspot mutants' interaction with ACE2 to understand the binding affinity and stability.

PMID: 34217923 2021 Virology

Introduction: VOC, 202012/01) with multiple S protein mutations (deletion 69-70, deletion 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) spread rapidly across South East England and London.