Structural Consequences of Variation in SARS-CoV-2 B.1.1.7.
PMID: 33969357
2021
Journal of cellular immunology
Method: The prefusion SARS-CoV-2 (Wuhan-Hu-1) spike glycoprotein with a single receptor-binding domain up (PDB 6VSB) was used as the basis for modeling D570A, D614G, T716I, S982A and D1118H.
Will Mutations in the Spike Protein of SARS-CoV-2 Lead to the Failure of COVID-19 Vaccines?
PMID: 33975397
2021
Journal of Korean medical science
Introduction: The UK SARS-CoV-2 B.1.1.7 variant is defined by multiple spike (S) protein changes (deletion 69-70, deletion 145, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H).
Implications of the Novel Mutations in the SARS-CoV-2 Genome for Transmission, Disease Severity, and the Vaccine Development.
Introduction: The B.1.1.7 variant-specific non-synonymous mutations and deletions have been detected in the spike protein including deletion 69-70, deletion 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H.
Convalescent-Phase Sera and Vaccine-Elicited Antibodies Largely Maintain Neutralizing Titer against Global SARS-CoV-2 Variant Spikes.
Introduction: The B.1.1.7 lineage (VOC-202012/01) variant identified in patients in the United Kingdom encodes a spike protein with 8 mutations in addition to D614G (Delta69-70, Y144Del, N501Y, A570D, P681H, T716I, S982A, and D1118H).
Result: A detailed analysis of two donor sera chosen at random showed that the sera neutralized B.1.1.7 and its constitutive point mutations similarly, with the exception of T716I, which was more easily neutralized than D614G.
Result: Analysis of the B.1.1.7 variant and its component mutations showed that the single point mutations had little effect on infectivity (Delta69-70, Y144Del,
Mutation in a SARS-CoV-2 Haplotype from Sub-Antarctic Chile Reveals New Insights into the Spike's Dynamics.
Discussion: Recently, the United Kingdom has reported that a large proportion of new cases in South East England belonged to a new single phylogenetic cluster defined by multiple spike protein mutations (deletions 69-70 and 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H).
Mutations in the B.1.1.7 SARS-CoV-2 Spike Protein Reduce Receptor-Binding Affinity and Induce a Flexible Link to the Fusion Peptide.
Abstract: The design principle can be rapidly adapted for other mutations (as shown also for E484K and T716I) highlighting the advantages of quick optimization and roll-out of CRISPR diagnostics (CRISPRDx) for disease surveillance even beyond COVID-19.
Method: A region from the SARS-CoV-2 S gene containing N501Y/T716I/E484K mutations was reverse transcribed and amplified using a single end 5' biotin-labeled primer.
Result: Notably, we were able to successfully validate RAY for two more mutations E484K and T716I
Figure: (G) RAY outcomes on E484K and T716I mutations from patient samples.
Proliferation of SARS-CoV-2 B.1.1.7 Variant in Pakistan-A Short Surveillance Account.
Abstract: Based on the partial sequencing of SGTF samples 93.5% (n = 29/31) showed the characteristic N501Y, A570D, P681H, and T716I mutations found in the B.1.1.7 variant.
Introduction: The B.1.1.7 harbors 17 amino acid changes mostly in the Spike (S) protein (69-70del, 144del, N501Y, A570D, P681H, T716I, S982A, and D1118H).
Result: Based on the partial sequencing of spike gene of SGTF samples, 93.5% (n = 29/31) showed the characteristic N501Y, PMID: 34146731
2021
Infection, genetics and evolution
Introduction: Among the mutations in the B.1.1.7, nine mutations, including H69-V70 deletion (Delta69/Delta70), Y144 deletion (Delta144), N501Y, A570D, D614G, P681H, T716I, S982A, and D1119H, were located in the viral spike protein.
ViMRT
SARS_CoV_2 mutation literature information.
PMID: 
2021
Journal of cellular immunology
