literature

SARS_CoV_2 mutation literature information.

Conformational Flexibility and Local Frustration in the Functional States of the SARS-CoV-2 Spike B.1.1.7 and B.1.351 Variants: Mutation-Induced Allosteric Modulation Mechanism of Functional Dynamics and Protein Stability.

PMID: 35163572 2022 International journal of molecular sciences

Result: In some contrast, mutations in the functional RBD positions (K417,

Result: Strikingly, the positions of mutational changes A570D, D614G, T716I, S982A in the S-B.1.1.7 states (Figure 5C,D) and positions D614G and A701V in the S-B.1.351 conformations (Figure 5E,F) are aligned with the local minima along slow mode displacement profiles and correspond to immobilized positions in slow motions.

Result: These results are also consistent with the experiments showing that N501Y, T716I and D1118H mutations induce only minimal local conformational changes without affecting stability of the S protein.

In silico analysis of mutant epitopes in new SARS-CoV-2 lineages suggest global enhanced CD8+ T cell reactivity and also signs of immune response escape.

PMID: 35149224 2022 Infection, genetics and evolution

Discussion: To date, there are no studies associating the nsSNVs in protein S such as D80A, P618H, T1027, and T716I with increased transmissibility or immune evasion, since most studies focused on nsSNVs located on the RBD.

Worldwide SARS-CoV-2 haplotype distribution in early pandemic.

PMID: 35171928 2022 PloS one

Result: However, p.Asn501Tyr variant and other 8 of the 17 variants (3267C>T p.Thr1001Ile, 6954T>C p.Ile2230Thr in the ORF1ab gene; 23063A>T, 23271 C>A p.Ala570Asp, 23604C>A p.Pro681His, 23709C>T p.Thr716Ile in the S gene; 27972C>T p.Gln27*, 28048G>T p.Arg52Ile in the Orf8 gene;

Genomic Diversity of SARS-CoV-2 in Algeria and North African Countries: What We Know So Far and What We Expect?

PMID: 35208920 2022 Microorganisms

Discussion: Comparative genomic analysis of SARS-CoV-2 genomes revealed multiple crucial mutations to the Spike gene including K417N, K417T, E484K, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H, which may aggravate the severity of SARS-CoV-2 more than the wild type variant, and potentially raise the concern of vaccine efficacy against novel strains.

Pan-SARS neutralizing responses after third boost vaccination in non-human primate immunogenicity model.

PMID: 35101265 2022 Vaccine

Table: T716I

SARS-CoV-2 Beta and Delta variants trigger Fc effector function with increased cross-reactivity.

PMID: 35233544 2022 Cell reports. Medicine

Method: The SARS-CoV-2 Wuhan-1 spike, cloned into pCDNA3.1 was mutated using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) and NEBuilder HiFi DNA Assembly Master Mix (NEB) to include D614G (original) or lineage defining mutations for Alpha (DEL69-70, DEL144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), Beta (L18F, D80A, D215G, 242-244del, K417N, E484K, N501Y,

Table: T716I

Temporal-Geographical Dispersion of SARS-CoV-2 Spike Glycoprotein Variant Lineages and Their Functional Prediction Using in Silico Approach.

PMID: 34700382 2021 mBio

Result: At least 3,776 S protein variants belonging to alpha (B.1.1.7) variant lineage were identified, in which all contain 2 deletion events (amino acids 69 to 70 and 144) and 7 mutations (N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H).

Result: Dual mutants containing D614G and other amino acid changes, including those under positive selection or observed in VOCs (L5F, L18F, S98F, W152L/C, E154K, L222V, and A262S in S1-

Evolution, Mode of Transmission, and Mutational Landscape of Newly Emerging SARS-CoV-2 Variants.

PMID: 34465019 2021 mBio

Table: T716I

SARS-CoV-2 Alpha, Beta, and Delta variants display enhanced Spike-mediated syncytia formation.

PMID: 34601723 2021 The EMBO journal

Result: Alpha S contains the 69/70 and Y144 deletions in the N-terminal domain (NTD), P681H and T716I mutations in the S1/S2 cleavage site, the S982A mutation in the heptad repeat 1 (HR1) site and the D1118H mutation in between HR1 and HR2.

Evaluation of the clinical and analytical performance of the Seegene allplex SARS-CoV-2 variants I assay for the detection of variants of concern (VOC) and variants of interests (VOI).

PMID: 34628158 2021 Journal of clinical virology

Table: T716I

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