Evaluation of a Rapid and Accessible Reverse Transcription-Quantitative PCR Approach for SARS-CoV-2 Variant of Concern Identification.
PMID: 35465708
2022
Journal of clinical microbiology
Abstract: We designed and analytically validated a two-reaction multiplex reverse transcription-quantitative PCR (RT-qPCR) assay targeting spike protein mutations L452R, E484K, and N501Y in reaction 1 and del69-70, K417N, and T478K in reaction 2.
Binding Interactions between Receptor-Binding Domain of Spike Protein and Human Angiotensin Converting Enzyme-2 in Omicron Variant.
PMID: 35481766
2022
The journal of physical chemistry letters
Abstract: Some of the OV RBD mutations are predicted to affect the antibody neutralization either through their role in the S-protein conformational changes, such as S371L, S373P, and S375F, or through changing its surface charge distribution, such as G339D, N440K, T478K, and E484A.
RBD-mRNA vaccine induces broadly neutralizing antibodies against Omicron and multiple other variants and protects mice from SARS-CoV-2 challenge.
Abstract: The RBD-mRNA-induced antibodies exerted moderate neutralization against authentic B.1.617.2 and B.1.1.529 variants, and pseudotyped B.1.351 and P.1 lineage variants containing K417N/T, E484K, and N501Y mutations, the B.1.617.2 lineage variant harboring L452R, T478K, and P681R mutations, and the B.1.1.529 lineage variant containing 38 mutations in the S protein.
Delta variant (B.1.617.2) of SARS-CoV-2: Mutations, impact, challenges and possible solutions.
PMID: 35507895
2022
Human vaccines & immunotherapeutics
Abstract: The enhanced transmissibility of Delta variant has been associated with critical mutations such as D614G, L452R, P681R, and T478K in the S-protein.
Linked nosocomial COVID-19 outbreak in three facilities for people with intellectual and developmental disabilities due to SARS-CoV-2 variant B.1.1.519 with spike mutation T478K in the Netherlands.
Discussion: An in silico molecular dynamics study on the protein structure of Spike has predicted that the T478K mutation, substituting a non-charged amino acid (Threonine) with a positive one (Lysine) may significantly alter the electrostatic surface of the protein (Figure 3), and therefore the interaction with ACE2, drugs, or antibodies, 25 and that the effect can be increased if combined by other co-occurring Spike mutations (see Table 1).
Discussion: Another experiment showed that T478K and T478R mutants were enriched when SARS-CoV-2 viral cultures were tested against weak neutralizing antibodies, 26 highlighting, at least in vitro, a possible genetic route the virus can follow to escape immune recognition.
Discussion: Everything considered, we believe that the continued genetical and clinical monitoring o
Vaccine-escape and fast-growing mutations in the United Kingdom, the United States, Singapore, Spain, India, and other COVID-19-devastated countries.
Result: 2 shows that T478K leads to the highest increase in ACE2-S protein RBD BFE change, indicating that fast-growing mutation T478K may potentially make the SARS-CoV-2 more transmissible and infectious.
Result: Among them, T478K is part of the Mexico variant B.1.1.222 and has the highest growth rate since late October 2020.
Result: From analyzing the SNP profiles in Mexico, we notice that 6 ACE2 binding-strengthening mutations, L452R, S477N, T478K, S494P, E484K, and A552V, have a rapid growth since late October 2020.
Result: However, T478K does not po
The Spike of Concern-The Novel Variants of SARS-CoV-2.
Introduction: The European Centre for Disease Prevention and Control (ECDC) lists currently the following sub-variants: B.1.617.1 was detected in India in December 2020 (L452R, E484Q, D614G, P681R, Q1071H (some viruses also carry V382L)), B.1.617.2 was initially identified in India in December 2020 but is now on the rise in the UK (T19R, Delta157-158, L452R, T478K, D614G, P681R, D950N), and the rare variant B.1.617.3 (T19R, Delta157-158, L452R, E484Q, PMID: 34147856
2021
Computers in biology and medicine
Table: T478K
Tracking SARS-CoV-2 Spike Protein Mutations in the United States (2020/01 - 2021/03) Using a Statistical Learning Strategy.
Introduction: It is characterized by spike mutations T19R, G142D, Delta157-158, L452R, T478K, D614G, P681R, and D950N (Figure 3).
Introduction: The impact on the immune escape capacity of three sublineages of B.1.617 is expected, owing to RBD mutations L452R, T478K, and E484Q and their combination with NTD mutations and deletions, particularly in the case of B.1.617.2.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Journal of clinical microbiology
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