Host Response to SARS-CoV2 and Emerging Variants in Pre-Existing Liver and Gastrointestinal Diseases.
PMID: 34760721
2021
Frontiers in cellular and infection microbiology
Abstract: The mutations in the spike protein of VOC are implicated for increased receptor binding (N501Y, P681R) and immune escape (L452R, E484K/Q, T478K/R) to host response.
Introduction: As of May 2021, three sublineages have been detected for this variant (B.1.617.1/2/3) with B.1.617.2 being the most dominant, having a unique T478K mutation absent in sublineages 1 and 3, serving as a possible route of immune evasion.
Postvaccination SARS-COV-2 among Health Care Workers in New Jersey: A Genomic Epidemiological Study.
Method: The SARS-CoV-2 target sequences include (i) the wild-type (WT) gene fragment of S protein (S; nucleotides [nt] 21,563 to 25,384; GenBank accession number MN908947); (ii) the mutant gene fragments of S protein, inclu
Table: T478K
Discussion: In our study, we developed a CRISPR-Cas12a-based genotyping assay and have approved its feasibility to detect single nucleotide mutations and to distinguish variants of SARS-CoV-2 based on the combination of a series of crRNAs that are specific for the most important and signature mutations, including K417N, L452R/Q, T478K, E484K/Q, and N501Y within the spike protein of SARS-CoV-2.
SARS-COV-2 Delta variant displays moderate resistance to neutralizing antibodies and spike protein properties of higher soluble ACE2 sensitivity, enhanced cleavage and fusogenic activity.
Abstract: The spike proteins of B.1.617.1, B.1.617.2, and AY.1 variants have several substitutions in the receptor binding domain (RBD), including L452R+E484Q, L452R+T478K, and K417N+L452R+T478K, respectively, that could potentially reduce effectiveness of therapeutic antibodies and current vaccines.
Linked nosocomial COVID-19 outbreak in three facilities for people with intellectual and developmental disabilities due to SARS-CoV-2 variant B.1.1.519 with spike mutation T478K in the Netherlands.
Abstract: Recently, SARS-CoV-2 delta variant prevails over different countries that have 3 unique mutation sites: E156del/R158G in the N-terminal domain and T478K in a crucial receptor binding domain.
Conclusion: It is great period to prepare a vaccine or a neutralizing antibody against SARS-CoV-2 delta variant to prevent another wave of SARS-CoV-2 pandemic although there is no experimental evidence to confirm the significance of the T478K mutation in the SARS-CoV-2 delta variant.
Conclusion: The analysis of mutation sites in the critical RBM of spike protein on the SARS-CoV-2 variants ascertained a distinct T478K mutation in the SARS-CoV-2 delta variant.
Table: T478K
Figure: The unique
Introduction and rapid dissemination of SARS-CoV-2 Gamma Variant of Concern in Venezuela.
PMID: 34800714
2021
Infection, genetics and evolution
Introduction: Delta VOC harbors several mutations, such as L452R and T478K, being the former associated with an increased transmission potential and reduced susceptibility to protective immunity, both at humoral and cellular level.
Mutation-Induced Long-Range Allosteric Interactions in the Spike Protein Determine the Infectivity of SARS-CoV-2 Emerging Variants.
Abstract: To address this question, here we have individually assessed the effects of SARS-CoV-2 variant-specific spike (S) protein receptor-binding domain (RBD
Introduction: Here, we have considered prominent spike receptor-binding domain (RBD) mutations that are commonly observed among the VOI/VOC variants (Table 1), namely, E484K, K417N, L452Q, L452R, N501Y, and T478K.
Introduction: Of these, T478K and K417N substitutions in the spike protein make it characteristically different from other VOIs.
A rigorous framework for detecting SARS-CoV-2 spike protein mutational ensemble from genomic and structural features.
PMID: 34806033
2021
Current research in structural biology
Result: Further, for the characteristic RBD mutations across VoCs (K417 T/N, L452R, S477N, T478K, E484K, and N501Y), we observed MTR scores between 0.68 and 0.91, suggesting these substitutions to be tolerant.
Result: Interestingly, five out of eight mutations of RBD are present in the epitope region, where L452Rand N501Y substitutions map to the RBD-loop epitope and S477N, T478K, and E484K are harboured in the RBD-ridge epitope.
Result: The RBD
Relative Consolidation of the Kappa Variant Pre-Dates the Massive Second Wave of COVID-19 in India.
Discussion: Furthermore, while retaining all the key mutations contained in the kappa variant, the spike protein of the delta variant contains only one additional mutation in the RBD domain (T478K).
Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms.
Discussion: Taking into account that curcumin exhibited inhibition against D614G strain, through different treatment strategies, this compound was evaluated against infection by the Delta variant which contains mutations in the spike protein (L452R, T478K, P681R, and D614G) associated with an increase in viral infectivity, transmissibility and pathogenicity in individuals infected with SARS-CoV-2 and a decrease in antibody-mediated neutralization.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Frontiers in cellular and infection microbiology
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