literature

Temporal-Geographical Dispersion of SARS-CoV-2 Spike Glycoprotein Variant Lineages and Their Functional Prediction Using in Silico Approach.

PMID: 34700382 2021 mBio

Result: Twelve signature amino acid mutations (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, and V1176F) were observed in the S protein as well as other consensus changes, including ORF1a (S1188L and K1795Q), ORF1b (P214L and E1164D), ORF3a (S253P),

PMID: 34737587 2021 Infection and drug resistance

Method: They are:

Result: The B.1.351 control showed mutation in E484K, D614G, A701V, K417N, N501Y, and L242_244L, B.1.1.7 showed mutations in D614G, delH69V70, N501Y, and Q27stop, the control B.1.617.1 showed mutation in E484Q, P681R, L452R, D614G, and P.1 showed mutations in E484K, D614G, K417T, N501Y, and T20N.

Table: T20N

Functional differences among the spike glycoproteins of multiple emerging severe acute respiratory syndrome coronavirus 2 variants of concern.

PMID: 34746689 2021 iScience

Result: The two additional potential N-linked glycosylation sites created by the T20N and R190S changes in the NTDs of P.1 and B.1.1.248 S1 glycoproteins likely account for the observed higher molecular weight.

Genomic surveillance reveals the detection of SARS-CoV-2 delta, beta, and gamma VOCs during the third wave in Pakistan.

PMID: 34726786 2021 Journal of medical virology

Result: The gamma variants harbors non-synonymous lineage defining mutations, ORF1a: K1795Q (NSP3: K977Q) (A5648C), ORF1a: del:11288:9 (NSP6: S106), S:T20N (C21621A), S:R19S (G22132T), S:K417T (A22812C), S:E484K (G23012A), S:N501Y (

Epidemiology of COVID-19: An updated review.

PMID: 34759999 2021 Journal of research in medical sciences

Table: T20N

Predominance of SARS-CoV-2 P.1 (Gamma) lineage inducing the recent COVID-19 wave in southern Brazil and the finding of an additional S: D614A mutation.

PMID: 34763050 2021 Infection, genetics and evolution

Result: The Gamma sequences generated herein presented the classical mutational signatures in spike glycoprotein L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I and V1176F, with some exceptions, as in the sample hCoV-19/Brazil/LMM53965 and hCoV-19/Brazil/LMM54004 that change a guanine for an adenine in 614 position (D614G D614A) and the hCoV-19/Brazil/LMM54029 that change a histidine for a glutamic acid in 655

A rigorous framework for detecting SARS-CoV-2 spike protein mutational ensemble from genomic and structural features.

PMID: 34806033 2021 Current research in structural biology

Result: For instance, L18F, T20N, P26S, D138Y, and R190S are present in P.1 lineage.

Result: For the characteristic NTD mutations across VoCs (L18F, T19R, T20N, D80A, and T95I), we observed the MTR score of0.738 s

Result: Specifically, L18F, T19R, and T20N are present in the N-terminus epitope, whereas G142D, W152 R/L/C, M153T, and F157 are present in the supersite beta-hairpin epitope.

The Development of SARS-CoV-2 Variants: The Gene Makes the Disease.

PMID: 34940505 2021 Journal of developmental biology

Introduction: Still, it has been suggested that the great variability in the NTD between these variants, with allosteric influences from other mutations (T20N, P26S, D138Y, R190S; Table 2), could contribute to the observed differences.

Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.

PMID: 34925381 2021 Frontiers in immunology

Introduction: The Gamma P.1 variant encodes an S protein with 12 mutations (L18F, T20N, P26S, D138Y, R190S, K417N/T, E484K, N501Y, D614G, H655Y, T1027I, and V1176F), two of which are in the RBD (E484K, and N501Y).

Re-emergence of Gamma-like-II and emergence of Gamma-S:E661D SARS-CoV-2 lineages in the south of Brazil after the 2021 outbreak.

PMID: 34789293 2021 Virology journal

Table: T20N

Discussion: This hypothesis also justifies the absence of other mutations in the S protein, such as S:T20N, in the Gamma-like-II lineage.