Result: In contrast to lysates containing N T205I alone or N WT, the sensitivity of the Quidel Sofia 2 antigen test for lysates containing N D399N mutation alone was reduced by 1000-fold (comparing the last dilution where all tests were positive), similar to lysates containing the N T205I/D399N double mutant.
Result: This corresponded to a difference of approximately 948 pg N T205I and 1.22 x 106 pg of N T205I/D399N (Table 4).
Result: Thus, the Abbott BinaxNOW test appears unaffected by the
Arginine methylation of SARS-Cov-2 nucleocapsid protein regulates RNA binding, its ability to suppress stress granule formation, and viral replication.
PMID: 34029587
2021
The Journal of biological chemistry
Discussion: Interestingly, the only variant in N protein is R203K;G204R in the B.1.17 strain and T205I in B.1.351 strain, suggesting the RGT sequence is an important regulatory site.
Mutation Signatures and In Silico Docking of Novel SARS-CoV-2 Variants of Concern.
Result: In addition, the SA-VOC has several aa substitutions in NSP2 (R4C), NSP3 (K837N), NSP12 (P323L), NSP13 (E168D), NSP14 (S28C), and NSP15 (P205L), NS3 (Q57H, S171L), E (P71L), N-protein (T205I) (Table 1).
Genetic Diversity of SARS-CoV-2 over a One-Year Period of the COVID-19 Pandemic: A Global Perspective.
Discussion: Additionally, other variants like S25L in NSP7, S194L, R203K, G204R, and T205I in nucleocapsid, T85I and I120F in NSP2, S477N in spike, and Q57H in ORF3a have also been confirmed by previous studies.
Emerging variants of concern in SARS-CoV-2 membrane protein: a highly conserved target with potential pathological and therapeutic implications.
Result: Another mutation carried by most isolates in this clade (98%) that is worth noting is N:T205I, because it is present in multiple VOCs including CAL.20C (B.1.429 and B.1.427) and B.1.351, and that M and N proteins are both important for viral assembly.
Result: The novel combination of M:I82T, the three signature Spike mutations (S:S494P, the S:P681H and S:T716I) from B.1.1.7, and the N:T205I mutation is therefore of particular concern.
Table: T205I
Structure-Function Analyses of New SARS-CoV-2 Variants B.1.1.7, B.1.351 and B.1.1.28.1: Clinical, Diagnostic, Therapeutic and Public Health Implications.
Result: The D3L and T205I, S235F mutations in the N protein occur outside the dimer and RNA interaction interfaces, in the unstructured regions in its NTD and linker regions, respectively (Figure S10).
Genomic surveillance of SARS-CoV-2 in the Republic of Congo.
PMID: 33737129
2021
International journal of infectious diseases
Table: T205I
Neutralising antibody escape of SARS-CoV-2 spike protein: Risk assessment for antibody-based Covid-19 therapeutics and vaccines.
Result: However, the FUBAR test showed that four sites (L3606F and G392D on ORF1ab, D936Y on S protein, T205I on N protein) were under episodic positive/diversifying selection with posterior probability >= 0.95 and log BF >= 25.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Journal of clinical virology
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