Method: The SARS-CoV-2 Wuhan-1
spike, cloned into pCDNA3.1 was mutated using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) and NEBuilder HiFi DNA Assembly Master Mix (NEB) to include
D614G (original) or lineage defining mutations for Alpha (DEL69-70, DEL144,
N501Y,
A570D,
D614G,
P681H,
T716I,
S982A,
D1118H), Beta (
L18F,
D80A,
D215G, 242-244del,
K417N,
E484K,
N501Y,