Discussion: Recently, the United Kingdom has reported that a large proportion of new cases in South East England belonged to a new single phylogenetic cluster defined by multiple spike protein mutations (deletions 69-70 and 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H).
Mutations in the B.1.1.7 SARS-CoV-2 Spike Protein Reduce Receptor-Binding Affinity and Induce a Flexible Link to the Fusion Peptide.
Introduction: This variant is characterized by several amino acid deletions and exchanges, with most of the protein-coding mutations found within the surface-anchored spike (S) protein of the virus: del69-70HV, del144Y, N501Y, A570D, D614G, P681H, T761I, S982A, D1118H (Figure S1a).
Method: These were N501Y, A570D, D614G, P681H, T716I, S982A and D1118H.
Molecular epidemiology of SARS-CoV-2 isolated from COVID-19 family clusters.
Introduction: The B.1.1.7 harbors 17 amino acid changes mostly in the Spike (S) protein (69-70del, 144del, N501Y, A570D, P681H, T716I, S982A, and D1118H).
Recent progress on the mutations of SARS-CoV-2 spike protein and suggestions for prevention and controlling of the pandemic.
PMID: 34146731
2021
Infection, genetics and evolution
Introduction: Among the mutations in the B.1.1.7, nine mutations, including H69-V70 deletion (Delta69/Delta70), Y144 deletion (Delta144), N501Y, A570D, D614G, P681H, T716I, S982A, and D1119H, were located in the viral spike protein.
Introduction: These mutations in this strain may enhance virus transmissibility and infectivity, including the deletion of residues 69-70 and 144 and the substitution of A570D, D614G, T716I, S982A, D1118H, P681H, K417N, K417T, E484 K and N501Y.
Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the Alpha variant B.1.1.7.
Method: The mutations (Delta69/70, Delta144, N501Y, A570D, D614G, P681H, S982A, T716I and D1118H or K417N, E484K and N501Y) were introduced by amplification with primers with similar Tm.
Result: In addition to RBD N501Y and NTD DeltaH69/V70, B.1.1.7 is defined by further S mutations across S2 (T716I, S982A, and D1118H) and S1 (DeltaY144, A570D, and
Analysis of SARS-CoV-2 variant mutations reveals neutralization escape mechanisms and the ability to use ACE2 receptors from additional species.
Method: The variant B.1.1.7 (GISAID: EPI_ISL_601443) was constructed with total of 9 mutations including 69-70del, 144del, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H.
Specific allelic discrimination of N501Y and other SARS-CoV-2 mutations by ddPCR detects B.1.1.7 lineage in Washington State.
Abstract: S gene target failures (SGTF) were subsequently assayed by RT-ddPCR to confirm four mutations within the S gene associated with the B.1.1.7 lineage: a deletion at amino acid (AA) 69-70 (ACATGT), deletion at AA 145, (TTA), N501Y mutation (TAT), and S982A mutation (GCA).
Method: All four targets are within the S gene domain: a deletion at amino acid (AA) 69-70 (ACATGT), deletion at AA 145, (TTA), N501Y mutation (TAT), and S982A mutation (GCA).
Result: For each sample, a total of nine mutations were found in the spike protein: H69-70-, Y144-, N501Y, A570D, D614G, P681H, PMID: 34206453
2021
Viruses
Introduction: The first variant Alpha or B.1.1.7 that raised global concerns about increased transmissibility and potential immune evasion harbors seven missense mutations (N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) and three deletions in spike (69/70del and 144del) (Figure 3).
SARS_CoV_2 mutation literature information.
PMID: 
2021
Viruses
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