Abstract: By contrast, Beta was outcompeted by Alpha and wt-S614G in hamsters and in mice expressing human ACE2.
Abstract: In hamsters, which support high viral replication levels, Alpha and wt-S614G showed similar fitness.
Abstract: In vivo, competition experiments showed a clear fitness advantage of Alpha over wt-S614G in ferrets and two mouse models-the substitutions in S were major drivers of the fitness advantage.
Abstract: Our study highlights the importance of using multiple models to characterize fitness of VOCs and demonstrates that Alpha is adapted for replication in the upper respiratory tract and shows enhanced transmission in vivo in restrictive models, whereas Beta does not overcome Alpha or wt-S614G in naive animals.
Abstract: Progenitor variant virus expressing
Efficacy of vaccination and previous infection against the Omicron BA.1 variant in Syrian hamsters.
Abstract: While the Moderna mRNA vaccine reduces viral loads in the respiratory tissues upon challenge with an early S-614G isolate, the vaccine efficacy is not as pronounced after infection with the Omicron variant.
Method: Early contemporary S-614D and S-614G isolates (SARS-CoV-2/UT-NCGM02/Human/2020/Tokyo and SARS-CoV-2/UT-HP095-1N/Human/2020/Tokyo, respectively) were described previously.
Method: The ELISA was performed using a recombinant S-614G protein with a C-terminal HIS-tag purified by using TALON metal affinity resin from Expi293F cells (Thermo Fisher Scientific).
Result: All animals had high IgG antibody titers against the S
Molecular epidemiology analysis of early variants of SARS-CoV-2 reveals the potential impact of mutations P504L and Y541C (NSP13) in the clinical COVID-19 outcomes.
PMID: 33798758
2021
Infection, genetics and evolution
Introduction: In the correlation analysis, variants of S 614G was associated with case fatality rates (CRF) and median CFR in 12 countries, and variants ORF1ab 4715 L and S 614G were reported correlated with fatality rates in 28 countries and 17 states of the United States.
Characterization of a new SARS-CoV-2 variant that emerged in Brazil.
Abstract: However, the effect was less pronounced against the P.1 variant than the S-614G strain.
Abstract: In addition, passive transfer of neutralizing antibodies to hamsters infected with the P.1 variant or the S-614G strain led to reduced virus replication in the lower respiratory tract.
Abstract: In contrast, the S-614D and S-614G strains were less well recognized than the P.1 variant by serum from a P.1-infected patient.
Abstract: Prior infection with S-614D or S-614G strains efficiently prevented the replication of the P.1 variant in the lower respirato
Ongoing global and regional adaptive evolution of SARS-CoV-2.
Figure: (D) The frequency of S 614G, at least one NLS-associated variant (N 194L, N119L, N203K, N205I, and N220V), and at least one emerging spike variant (SI Appendix.
Entropy based analysis of SARS-CoV-2 spread in India using informative subtype markers.
Abstract: All the glycosylation sites with altered patterns were in the spike head while the glycosylation of three sites in the stalk remained unchanged between S-614G and S-614D proteins.
Abstract: In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.
Abstract: The data showed that half of the N-glycosylation sequons changed their distribution of glycans in the S PMID: 32699345
2020
Journal of human genetics
Abstract: In BCG-vaccinated countries, the frequency of the S 614G variant had a trend of association with the higher fatality rate.
Result: Among the mutations we analyzed, the frequencies of ORF1ab 4715L-type and S 614G-type viruses showed significant positive correlations with fatality rates (Pearson's correlation coefficient (r) = 0.41, P = 0.029 and r = 0.43, P = 0.022, respectively.
Result: However, the significant correlations became not statistically significant after adjusted by the frequency of S 614G variant in multiple regression (P = 0.13 for HLA-A*11:01, P = 0.73 for HLA-A*02:06 and P = 0.45 for HLA-B*54:01).
Result: Since the clusters 2 and 3 were separated mainly by the frequency of N 203K/204R, we also examined the correlations of this variant or S
BioAider: An efficient tool for viral genome analysis and its application in tracing SARS-CoV-2 transmission.
PMID: 32904401
2020
Sustainable cities and society
Discussion: We found that mutant strains with NSP12-323L & S-614G were popular in the world and owned more than half the frequency (52 %) in the population, indicating that this mutant was dominant in SARS-CoV-2.
SARS-CoV-2 spike D614G variant confers enhanced replication and transmissibility.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Nature
Browser Board
Co-occurred Entities
Filtrator
ViMRT