Introduction: To assess the neutralization breadth of RBD-NP-elicited Abs, we evaluated serum neutralizing activity against a panel of pseudotyped viruses comprising wild-type (D614G) SARS-CoV-2 S and nine single-residue SARS-CoV-2 RBD mutants detected in clinical isolates (G446S, Y453F, L455F, T478I, E484A/K, F486L, S494P, and N501Y) as well as the B.1.1.7 (H69-V70 deletion, Y144 deletion, N501Y, A570D, P681H, T716I, PMID: 33804556
2021
Viruses
Abstract: Of concern are immune escape mutations acquired by the VOC: E484K, F490S, S494P (in the receptor binding motif of spike) and Q677H, Q675H (in the proximity of the polybasic cleavage site at the S1/S2 boundary).
Result: S494P.
Result: The VOC-202012/01 strain spike RBM mutations of special concern are substitutions E484K and S494P.
Result: The third most prevalent mutation, that is also by far the most prevalent RBM as well as in whole RBD mutation, is S494P (441 genomes).
Discussion: As the global spread of the VOC strain is very likely
Complete map of SARS-CoV-2 RBD mutations that escape the monoclonal antibody LY-CoV555 and its cocktail with LY-CoV016.
Result: The escape mutations present at the highest frequency among the sequenced isolates are E484K, L452R, and S494P for LY-CoV555 and K417N/T for LY-CoV016.
A new SARS-CoV-2 lineage that shares mutations with known Variants of Concern is rejected by automated sequence repository quality control.
Conclusion: However, two B.1.x sequences isolated from travelers returning to the UK also contain E484K, and the effect of S494P is not as well characterized.
Result: S494P is also located within the ACE2 receptor binding domain and experimental evidence suggests that mutations at this position decrease antibody binding affinity.
Result: Specifically, each sample contains Spike mutations S494P, N501Y, D614G, P681H, K854N, and E1111K.
Revealing the threat of emerging SARS-CoV-2 mutations to antibody therapies.
Abstract: We unveil, for the first time, that high-frequency mutations R346K/S, N439K, G446V, L455F, V483F/A, E484Q/V/A/G/D, F486L, F490L/V/S, Q493L, and S494P/L might compromise some of mAbs in clinical trials.
Mutations in the SARS-CoV-2 spike protein modulate the virus affinity to the human ACE2 receptor, an in silico analysis.
Result: The novel combination of M:I82T, the three signature Spike mutations (S:S494P, the S:P681H and S:T716I) from B.1.1.7, and the N:T205I mutation is therefore of particular concern.
Result: There were 10 other missense mutations present in at least 90% of the isolates in this clade and 8 of them were enriched by 73 to 146 fold compared to the general B.1 lineage including the 3 signature mutations in the spike protein (S:S494P, the S: Table: S494P
Vaccine-escape and fast-growing mutations in the United Kingdom, the United States, Singapore, Spain, India, and other COVID-19-devastated countries.
Abstract: A list of most likely vaccine escape mutations is given, including S494P, Q493L, K417N, F490S, F486L,
Conclusion: We report that rapidly growing mutations S494P, Q493L, K417N, F486L, F490S, R403K, E484K, K417T, L452R, E484Q, A475S, and F490L are more likely to disrupt existing vaccines and many antibody drugs.
Table: S494P
An Epidemiological Analysis of SARS-CoV-2 Genomic Sequences from Different Regions of India.
Discussion: In addition, this study observed the presence of individual amino acid variants in the SARS-CoV-2 variants B.1.1.7 (S494P), B.1.525 (A67V, Q677H), B.1.526 (L5F, T95I, S477N), and P2 (V1176F) in the earlier samples.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Heliyon
Browser Board
Co-occurred Entities
Filtrator
ViMRT