Prolonged shedding of infectious viruses with haplotype switches of SARS-CoV-2 in an immunocompromised patient.
PMID: 35430092
2022
Journal of infection and chemotherapy
Abstract: Seven haplotypes were identified and the non-synonymous mutations accumulated in the spike gene which included E484Q and S494P.
Conclusion: From haplotype 4 through haplotype 7, diversity was observed mainly in the S gene, and two of them (E484Q and S494P) were additional mutations in the receptor-binding motif of the receptor-binding domain (RBD).
Influence of treatment with neutralizing monoclonal antibodies on the SARS-CoV-2 nasopharyngeal load and quasispecies.
PMID: 34537363
2022
Clinical microbiology and infection
Introduction: The full spectrum of key spike mutations associated with resistance to mAbs is not yet established, but mutations K417N, E484D/K/Q, Q493R/K and S494P seem to be involved in virus escape and resistance to mAbs.
Discussion: However, this study only looked for mutations E484K/Q, F490S and S494P.
E484K and N501Y SARS-CoV 2 spike mutants Increase ACE2 recognition but reduce affinity for neutralizing antibody.
PMID: 34915409
2022
International immunopharmacology
Introduction: Later, it was demonstrated that the S494P caused a 3-5 fold decrease in neutralization titer and was reported in many cases in UK, USA, and Mexico.
Introduction: Using a large-scale genomic screening pipeline, my group previously identified two spike mutants, V367F and S494P, with enhanced human ACE2 binding ability.
Table: S494P
Emergence of two distinct variants of SARS-CoV-2 and an explosive second wave of COVID-19: the experience of a tertiary care hospital in Pune, India.
Modeling SARS-CoV-2 spike/ACE2 protein-protein interactions for predicting the binding affinity of new spike variants for ACE2, and novel ACE2 structurally related human protein targets, for COVID-19 handling in the 3PM context.
Abstract: By using our pipeline, we built 3D comparative models of the SARS-CoV-2 spike RBD/ACE2 protein complexes for the VoC B.1.1.7-United Kingdom (carrying the mutations of concern/interest N501Y, S494P, E484K at the RBD), P.1-Japan/Brazil (RBD mutations: K417T, E484K, N501Y), B.1.351-South Africa (RBD mutations: K417N, E484K, N501Y), B.1.427/B.1.429-California (RBD mutations: L452R), the B.1.141 (
Aggregation of high-frequency RBD mutations of SARS-CoV-2 with three VOCs did not cause significant antigenic drift.
Result: Among the possible Beta variants, the Beta+V367F pseudovirus variant showed nearly fourfold enhanced infectivity, especially in Huh7 and LLC-MK2 cells, and Beta+S494P and Beta+A520S showed similar effects (Figure 2B).
Result: Effect of S494P, A520S, and V367F substitutions on spike protein expression on pseudotyped virus and cell-cell fusion.
Result: Furthermore, among the results from 293T-ACE2 cells of different species that were zoophilic, three mutant variants:S494P, V367F, and A520S:were particularly enhanced in infectivity in cells expressing mouse ACE2, reaching or approaching four-fold (Figure S1B), and these mutant va
Mutations in the receptor-binding domain of human SARS CoV-2 spike protein increases its affinity to bind human ACE-2 receptor.
PMID: 35109768
2022
Journal of biomolecular structure & dynamics
Abstract: Here, we use the crystal structure of the RBD in complex with ACE-2 available in the public domain and analyse the 250 ns molecular dynamics (MD) simulations of wild-type and mutants; K417N, K417T, N440K, N501Y, L452R, T478K, E484K and S494P.
Tracking cryptic SARS-CoV-2 lineages detected in NYC wastewater.
Neutralisation Hierarchy of SARS-CoV-2 Variants of Concern Using Standardised, Quantitative Neutralisation Assays Reveals a Correlation With Disease Severity; Towards Deciphering Protective Antibody Thresholds.
Result: First, the 10 most observed or fast-growing RBD mutations are N501Y, L452R, T478K, E484K, K417T, S477N, N439K, K417N, F490S, and S494P, as shown in Table 1.
Result: Furthermore, high-frequency 2 comutation sets [E484K, N501Y], [F490S, N501Y], and [S494P, N501Y] are all considered to be the emerging variants that have the potentia
SARS_CoV_2 mutation literature information.
PMID: 
2022
Journal of infection and chemotherapy
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