Role of the Microbiome in the Pathogenesis of COVID-19.
PMID: 35433495
2022
Frontiers in cellular and infection microbiology
Table: S477N
Inhibitor screening using microarray identifies the high capacity of neutralizing antibodies to Spike variants in SARS-CoV-2 infection and vaccination.
Result: The most prevalent D614G mutation and some mutations of interest (i.e., N501Y, L452R, K417N, N439K, S477N, S494P) were among the variants printed.
Discussion: The S variants tested here contain mutations that were selected from the COVID-19 virus mutation tracker database and literature, including known mutations like N501Y, L452R, K417N, N439K, S477N and S494P, and less publicized mutations (Figure S2).
SARS-CoV-2 BA.1 variant is neutralized by vaccine booster-elicited serum, but evades most convalescent serum and therapeutic antibodies.
PMID: 35380448
2022
Science translational medicine
Result: We compared the neutralization titers of these serum samples against pseudoviruses bearing spike proteins from the following variants: D614G, Omicron (A67V, del69-70, T95I, del142-144, Y145D, del211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R,
Mutational cascade of SARS-CoV-2 leading to evolution and emergence of omicron variant.
Result: All SARS-CoV-2 genomes detected showed that the virus belonged to the clade 20A.EU2, with the presence of the characteristic S477N and the D614G mutations on the spike protein.
Circulation of SARS-CoV-2 Variants among Children from November 2020 to January 2022 in Trieste (Italy).
Discussion: As for N439K, S477N occurs in the RBD region and seems to confer enhanced binding ability to the ACE2 receptor, but it does not seem to be associated with the loss of neutralizing activities of convalescent serum antibodies.
Discussion: In our cohort, and compared to the D614G strain, the S477N viral load was slightly lower, while no difference in the cl
Discussion: In our study, the S477N substitution was detected in one sample, with a frequency of 3.1%, resembling its frequency rate in Europe, which ranges from 4 to 7%.
Discussion: The variant carrying the S477N substitution spread in some European countries, with a higher prevalence in France during the summer of 2020 and a frequency of detection ranging from 4-7%.
Omicron Variant of SARS-CoV-2 Virus: In Silico Evaluation of the Possible Impact on People Affected by Diabetes Mellitus.
Discussion: This variation, together with several mutations on Spike-RBD that were linked to an increased affinity for ACE2 (such as S477N, Q498R, and N501Y), is predicted to contribute to an increase in the interactivity between Omicron-Spike-RBD and ACE2 in a hyperglycemic environment.
Human serum from SARS-CoV-2-vaccinated and COVID-19 patients shows reduced binding to the RBD of SARS-CoV-2 Omicron variant.
Discussion: S477N, T478K, and E484A were initially at ~47% (status 2021-12-14, 2146 sequences), now instead above 88%, as N501Y (status 2022-02-07, 873.492 sequences).
Discussion: Several Omicron RBD mutations are assumed to increase the binding to ACE2: G339D, S477N, T478K, Q493K, and N501Y; others are proposed to be neutral: S371L, S373P, G446S, E484A, Q493R, and Q498R, or are assumed to reduce the binding to ACE2: PMID: 35233566
2022
Research square
Abstract: To build an investigative framework, we have applied an unsupervised machine learning approach to 4296 Omicron viral genomes collected and deposited to GISAID as of December 14, 2021, and have identified a core haplotype of 28 polymutants (A67V, T95I, G339D, R346K, Introduction: Most of these mutations (G339D, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H) present on the RBD surface.
SARS_CoV_2 mutation literature information.
PMID: 
2022
MedComm
Browser Board
Co-occurred Entities
Filtrator
ViMRT