Result: Binding of AX290ch was reduced, most likely due to the mutation S477N.
Result: The alternative substitution S477N that is more widespread in circulating viruses (1.482%) was not found in the sequenced viral genomes.
Result: We have performed a test of binding of chimeric antibodies AX677ch and AX290ch to the ectodomain trimer of the Omicron S protein (carrying mutations A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K,
The significant immune escape of pseudotyped SARS-CoV-2 variant Omicron.
Result: There are 32 mutations on the Spike of Omicron, including the following sites: A67V, H69del-V70del, T95I, G142D-V143del-Y144del-Y145del, N211del-L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, PMID: 34915409
2022
International immunopharmacology
Introduction: G446V RBD mutation was reported to reduce ACE2 binding affinities, but S477N mutation strengthen the binding between SARS-CoV2 spike RBD and hACE2.
Introduction: On the otherhand, G446V, S477N, G485R, and F490S RBD mutants demonstrated ~3-5 fold decrease in neutralization titer for few sera.
Table: S477N
Efficacy of mRNA, adenoviral vector, and perfusion protein COVID-19 vaccines.
Result: There were also three sequences carrying D138Y+ S477N+ D614G triple mutations sampled from shiraz and Tehran.
Discussion: L452R, T478K, N501Y and S477N have been the most frequently detected mutations in RDB.
Discussion: Notably, N501Y and S477N has also been reported as frequent mutations in this domain in a World-wide assay.
Discussion: The mutations D138Y and S477N are the same as those found in the NTD of VOC Gamma (P.1) and RBD of VOI of lota, respectively.
Discussion: This sequence also had an additional mutation
Modeling SARS-CoV-2 spike/ACE2 protein-protein interactions for predicting the binding affinity of new spike variants for ACE2, and novel ACE2 structurally related human protein targets, for COVID-19 handling in the 3PM context.
Abstract: By using our pipeline, we built 3D comparative models of the SARS-CoV-2 spike RBD/ACE2 protein complexes for the VoC B.1.1.7-United Kingdom (carrying the mutations of concern/interest N501Y, S494P, E484K at the RBD), P.1-Japan/Brazil (RBD mutations: K417T, E484K, N501Y), B.1.351-South Africa (RBD mutations: K417N, E484K, N501Y), B.1.427/B.1.429-California (RBD mutations: L452R), the B.1.141 (
The Role of Spike Protein Mutations in the Infectious Power of SARS-COV-2 Variants: A Molecular Interaction Perspective.
Conclusion: The S477N mutation leads to two N477(S) S19(ACE2) persistent primary hydrogen bonds.
Conclusion: To summarize, we offer a dissection of the explicit residue to residue intermolecular interactions resulting from the specific S477N, N501Y, K417N, K417T, E484K mutations in the Method: S477N: The extended pairs were extracted from a snapshot of the MD simulations carried out by Singh and co-workers.
Figure: S477N mutation.
Discussion: One serine asparagine mutation, S477N (Table 1, Figure 1), was found in the bonding motif of the RBD.
Longitudinal analysis of SARS-CoV-2 spike and RNA-dependent RNA polymerase protein sequences reveals the emergence and geographic distribution of diverse mutations.
PMID: 34801754
2022
Infection, genetics and evolution
Result: 4), and S477N was observed in more than 90% sequences from Oceania between the week of July 15th and the week of August 16th.
Result: first detected in New York (L5F, T95I, D253G; E484K or S477N; D614G, and A701V), appear together consistently after the week of October 4th, 2020, though the fact that many of these mutations are shared with other phylogenetic lineages makes it difficult to co
Figure: 1 associated with the Iota variant of interest are L5F, D253G, E484K, S477N, D614G, A701V).
SARS_CoV_2 mutation literature information.
PMID: 
2022
Nature
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