Result: However, as previously demonstrated, the virus evolves in cell culture and a single change in the S coding region (S247R) was already detected in over 40% of the viral population (Table 2).
Comprehensive annotations of the mutational spectra of SARS-CoV-2 spike protein: a fast and accurate pipeline.
PMID: 32954666
2021
Transboundary and emerging diseases
Table: S247R
IgV somatic mutation of human anti-SARS-CoV-2 monoclonal antibodies governs neutralization and breadth of reactivity.
Method: Alternatively, lentivirus Lenti-GF1-SARS-CoV-2Delta19AA truncated spike envelope displaying the H49Y, S247R, V367F, R408I, V483A, H519Q, A520S, and D614G mutations was produced using the same method.
Result: While plasma 3 exhibited broad neutralization, plasma from subject 102 did not neutralize at all viruses pseudotyped with the Wuhan-1, D614G, S247R, or H49Y spikes but neutralized moderately H519Q, and A520S spike
Result: We used biolayer interferometry (BLI) to measure the affinity of the RBD-binding antibodies and found that compared to Victoria (SARS-CoV-2/human/AUS/VIC01/2020), an early isolate of SARS-CoV-2, which has a single change S247R in S compared to the Wuhan strain, mAb binding was significantly impacted, with a number showing complete knockout of activity (Figure 2I).
SARS-CoV-2 mutations: the biological trackway towards viral fitness.
Evidence of a putative glycosaminoglycan binding site on the glycosylated SARS-CoV-2 spike protein N-terminal domain.
PMID: 33968333
2021
Computational and structural biotechnology journal
Result: It is this flexibility that allows the bridging of the two regions (furin cleavage site and the 245H-S247R region), in a manner similar to what was observed in the studies by Perkins and Rashid.
Result: Of particular interest was the S247R mutation, which is physically located close to the PRRARS furin cleavage site and close to some of the deletions in the UK mutant strain (B.1.1.7) (Table S5).
Result: Only HS bridges the gap between the furin site and S247R, while HP sits in the pocket between the PRRARS loop and the loop containing R634.
Result: The putative binding site at 245H-S247R did not
Result: Using GROMACS, we ran MD simulations with the HS and HP dodecasaccharides to conduct an in depth analysis of the binding to the mutated site, S247R, and the PRRARS furin cleavage site.
Bioinformatics Analysis Unveils Certain Mutations Implicated in Spike Structure Damage and Ligand-Binding Site of Severe Acute Respiratory Syndrome Coronavirus 2.
PMID: 34121839
2021
Bioinformatics and biology insights
Table: S247R
Functional and Structural Characterization of SARS-Cov-2 Spike Protein: An In Silico Study.
PMID: 34158771
2021
Ethiopian journal of health sciences
Table: S247R
Emerging mutation in SARS-CoV-2 spike: Widening distribution over time in different geographic areas.
Discussion: We found 17 mutation types of total sequences located within the NTD including T22I, P25L, T29I, Y38C, H49Y, S50L, F86S, T95I, E96G, S151G, N185K, N188K, V213L, S221W, S247R, G261D and Y279N.
Mutational analysis in international isolates and drug repurposing against SARS-CoV-2 spike protein: molecular docking and simulation approach.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Viruses
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