Introduction: In addition, an Nsp12 mutation (P323L) and N protein double mutations (R203K and G204R) were also found.
Result: In addition, these mutations were expressed as amino acid changes in the spike (D614G), Nsp12 (P323L), and N proteins (R203K and G204R).
Discussion: Siqi suggested that R203K and G204R mutations could change viral protein structure, binding affinity, and hot spots of the interface, thereby impact on SARS-CoV-2 transmission, diagnosis, and treatment of PMID: 35004593
2021
Frontiers in public health
Result: As showed in Figure 3, two amino acid substitutions occurred in N gene, such as p.Gli204Arg substitution in 46 SARS-COV-2 samples (16%) and p.Arg203Lys in 46 SARS-COV-2 samples (17%).
The Rapid Antigen Detection Test for SARS-CoV-2 Underestimates the Identification of COVID-19 Positive Cases and Compromises the Diagnosis of the SARS-CoV-2 (K417N/T, E484K, and N501Y) Variants.
Discussion: These data further suggest that this rapid antigen test could also fail to detect other variants, such as delta (B.1.617.2), because, in addition of Spike protein mutations, the delta variant also has mutations in the N protein (different to R203K).
Discussion: This kind of detection can explain the low capacity of RAT to detect the variants, which has the substitutions P80R and R203K in the N protein.
Omicron: A Heavily Mutated SARS-CoV-2 Variant Exhibits Stronger Binding to ACE2 and Potently Escapes Approved COVID-19 Therapeutic Antibodies.
Introduction: Omicron also had mutations in the other structural proteins, including Envelope (E) (T9I), Membrane (M) (D3G, Q19E, and A63T), and Nucleocapsid (N) (P13L, Delta31-33, R203K, G204R), further enhancing their infectivity.
Mutations in SARS-CoV-2 viral RNA identified in Eastern India: Possible implications for the ongoing outbreak in India and impact on viral structure and host susceptibility.
Figure: (B) 28881-3 GGG/AAC (R203K and G204R)) mutations in the nucleocapside protein coding gene in Clustal Omega.
Figure: (Bottom) This panel shows the domain organization of the variant SARS-CoV-2 N-protein with mutations at position 203 and 204 (R203K, G204R).
Discussion: S2, S3 and S5, shared rare set of three contiguous mutations in their genome which resulted in the consecutive alterations of R203K and G204R.
Discussion: We also analysed the predicted structural alterations in the viral nucleocapsid protein, which might be caused by consecutive alterations of R203K and G204R.
SARS-CoV-2 genomic surveillance in Taiwan revealed novel ORF8-deletion mutant and clade possibly associated with infections in Middle East.
Result: We further found 4 Taiwanese strains (CGMH-CGU-12, -13, -18, and -20, top four in Figure 3E) shared the same N gene mutations R203K and G204R resulting from 3 nucleotide mutations G608A, G609A, and G610C.
Identification of the nucleotide substitutions in 62 SARS-CoV-2 sequences from Turkey.
Discussion: These mutations were due to nucleotide substitutions in 3 nucleotides in order where 2 of them (G28881A and G28882A) results in arginine to lysine (R203K) substitution and the third one (G28883C) results in glycine to arginine (G204R) in the nucleocapsid phosphoprotein, where both substituted amino acids differ from their original amino acids in means of their isoelectric points (Pachetti et al., 2020) (Table 1).
SARS-CoV-2 genomic variations associated with mortality rate of COVID-19.
Result: In the cluster 2, K/R variants of N protein R203K/G204R mutations were significantly enriched at 43.1%, compared with the other clusters (5.2%, P = 0.00011 for the cluster 1 and 11.8%, P = 5.6 x 10-7 for the cluster 3; Supplementary.
Molecular Epidemiology Analysis of SARS-CoV-2 Strains Circulating in Romania during the First Months of the Pandemic.
SARS_CoV_2 mutation literature information.
PMID: 
2021
PloS one
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