Result: Twelve signature amino acid mutations (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, and V1176F) were observed in the S protein as well as other consensus changes, including ORF1a (S1188L and K1795Q), ORF1b (P214L and E1164D), ORF3a (S253P), PMID: 34730486
2021
Microbial genomics
Result: The most frequent substitutions among sequences were L71F (22.6 %) in the NSP7; P303L (99 %) in the RNA-dependent RNA polymerase (RdRp); D614G (98.5 %) and V1176F (41%) in the spike; I33T (37.4 %) in the ORF6; R203K (93.5 %), G204R (93.8 %), and I292T (39 %) in the N protein (Table S1).
Discussion: Additionally, the N protein also showed positive selection pressure in the sites S202C/I and
Emerging Severe Acute Respiratory Syndrome Coronavirus 2 Mutation Hotspots Associated With Clinical Outcomes and Transmission.
Result: D614G increased from 74.6% to 99.9%; T265I, Q57H, R203K, and G204R started to increase in March and gradually decreased in July (Figure 3D).
Discussion: Some of the mutation hotspots identified herein, namely, R203K, G204R, L3930F, and V1176F, were also high in severe cases, but significant statistical differences were not found.
Discussion: The R203K and G204R variants co-occurred in the N protein and caused dramatic changes in protein structure [root mean square deviation (RMSD) >=5.0 A], thus decreasing the flexibility of the domain.
Analysis of 329,942 SARS-CoV-2 records retrieved from GISAID database.
PMID: 34735950
2021
Computers in biology and medicine
Discussion: Also, R203K and G204R in the N gene were found to occur together with high frequency, which is confirmed in our research.
Discussion: Four mutations with a frequency greater than 20% featured the N gene: G28881A (R203K), G28882A (R203R), G28883S (R203R), and C28932T (A220V).
Discussion: Interestingly, Q57H and R203K were found to cause substantial changes in protein structures (RMSD >=5.0 A).
Emergence of B.1.524(G) SARS-CoV-2 in Malaysia during the third COVID-19 epidemic wave.
Genome-wide identification and prediction of SARS-CoV-2 mutations show an abundance of variants: Integrated study of bioinformatics and deep neural learning.
PMID: 34812411
2021
Informatics in medicine unlocked
Discussion: Along with the previously found GGG > AAC mutation, our mutational type analysis identify some another multi-nucleotide mutations CC > TT, TG > CA, and AT > TA which are in the top 20 mutational type and should be monitored for the future as the GGG > AAC (R203K and G204R) reported to be associated with the insertion of a lysine in SR domain of N protein which might affect the phosphorylation.
Nucleocapsid mutations R203K/G204R increase the infectivity, fitness, and virulence of SARS-CoV-2.
Figure: (D) IFs of 203K/204R (orange) and R203K/G204 (gray) among continents.
Figure: (G-I) show that the R203K/G204R substitutions enhance SARS-CoV-2 replication in primary human airway tissues.
Figure: Changes in the IF of 8 lineages including A222V (LG_4), N510Y (LG_5), and R203K/G204R (LG_3) and the distribution of lineages in the phylogenetic tree of all strains (H).
Figure: Effects of R203K/G204R on the severity of disease.
Figure: Evidence showing the adaptation of R203K/G204R.
Figure: Graphical overview of virology experiments to evaluate the impact of
Phylogenomics and population genomics of SARS-CoV-2 in Mexico during the pre-vaccination stage reveals variants of interest B.1.1.28.4 and B.1.1.222 or B.1.1.519 and the nucleocapsid mutation S194L associated with symptoms.
Result: Overall, we found that most of the genomic variants (SNVs+indels) occur in low frequencies, except for a few point variants, such as P323L in the RdRp protein, E484K, D614G and V1176F spanning the spike protein region, and R203K and S194L in the nucleocapsid region.
SARS-CoV-2 genetic variations associated with COVID-19 pathogenicity.
SARS_CoV_2 mutation literature information.
PMID: 
2021
mBio
Browser Board
Co-occurred Entities
Filtrator
ViMRT