Molecular characterization of SARS-CoV-2 from Bangladesh: implications in genetic diversity, possible origin of the virus, and functional significance of the mutations.
Result: Beside these, two of the high frequency (86%) SNVs (R203K and G204R) occurred in COVID-19 diagnostic RT-PCR target and B-Cell predicted epitope regions, of which the later was predicted to cause altered function of the Table: R203K
Discussion: The second most frequent mutation in our analysis was a tri-nucleotide change resulting in two amino acid changes which are R203K and G204R in N protein.
Discussion: This finding is similar with other studies those reported R203K and G204R destabilizes N protein structure but may enhance interaction with SARS-CoV-2 Envelop protein that may promote viral release.
Emergence and spread of the potential variant of interest (VOI) B.1.1.519 of SARS-CoV-2 predominantly present in Mexico.
Novel Nested-Seq Approach for SARS-CoV-2 Real-Time Epidemiology and In-Depth Mutational Profiling in Wastewater.
PMID: 34445204
2021
International journal of molecular sciences
Result: Although these mutations are located on the linker region (LKR) of the nucleocapsid phosphoprotein, which spans from position 175-254aa, only R203K belongs to the LKR's crucial Ser/Arg (SR)-rich motif that contains putative phosphorylation sites.
Result: Five well-characterized missense mutations, D614G (23403A>G)-S gene, Q57H (25563G>T)-ORF3a gene, P323L (14408C>T):ORF1ab/RdRP gene, R203K (28881G>A):N gene, and
Coding-Complete Genome Sequences of 11 SARS-CoV-2 B.1.1.7 and B.1.351 Variants from Metro Manila, Philippines.
Abstract: In addition, a retention of Q57H (B.1.X), R203K/G204R (B.1.1.X), T85I (B.1.2-B.
Result: Other major mutations noted are Q57H (26.5%), R203K/G204R (33%), G15S (12%), I120F (11.5%), and T85I (14%).
Result: These are Q57H (nucleotide 25,563, G to T) in ORF3a, the R203K + G204R combination (nucleotide 28,881, GGG to AAC) in nucleocapsid, and T85I (nucleotide 1,059, C to T) in ORF1a.
Multilevel systems biology analysis of lung transcriptomics data identifies key miRNAs and potential miRNA target genes for SARS-CoV-2 infection.
PMID: 34157472
2021
Computers in biology and medicine
Result: We have also noticed few other shared variations like R203K and G204R in nucleocapsid protein among the viral strains from Saudi Arabia and Russia, and the S166A variation in the viral strains from Saudi and India.
Table: R203K
Emerging Severe Acute Respiratory Syndrome Coronavirus 2 Mutation Hotspots Associated With Clinical Outcomes and Transmission.
Result: D614G increased from 74.6% to 99.9%; T265I, Q57H, R203K, and G204R started to increase in March and gradually decreased in July (Figure 3D).
Discussion: Some of the mutation hotspots identified herein, namely, R203K, G204R, L3930F, and V1176F, were also high in severe cases, but significant statistical differences were not found.
Discussion: The R203K and G204R variants co-occurred in the N protein and caused dramatic changes in protein structure [root mean square deviation (RMSD) >=5.0 A], thus decreasing the flexibility of the domain.
Temporal landscape of mutational frequencies in SARS-CoV-2 genomes of Bangladesh: possible implications from the ongoing outbreak in Bangladesh.
Result: Moreover, several mutations were found that persisted, (I120F, T412I, L37F, P323L, G204R, R203K, and D614G) for more than 6 weeks (Table 1 and.
Discussion: On the other hand, the temporal analysis of mutation accumulation also showed mutations (P323L, I120F, D614G, R203K, G204R) that persist over a longer period of time.
High-precision and cost-efficient sequencing for real-time COVID-19 surveillance.
Result: Ten of the 25 sequences shared additional mutations of R203K and G204R in the N (nucleocapsid) protein (skyblue circle in.
Table: R203K
Discussion: In addition to the G clade's other common mutation, NSP12-P323L, frequent mutations we observed include N-S194L, N-R203K, N-G204R, NSP2-T85I, and ORF3a-Q57H.
SARS_CoV_2 mutation literature information.
PMID: 
2021
bioRxiv
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