Result: These changes included, but were not limited to, D614G in Spike; R203K and G204K in N; and an out-of-frame deletion of five nucleotides in ORF7A, leading to its premature truncation.
RT-LAMP assay for rapid detection of the R203M mutation in SARS-CoV-2 Delta variant.
Generation of a Novel SARS-CoV-2 Sub-genomic RNA Due to the R203K/G204R Variant in Nucleocapsid: Homologous Recombination has Potential to Change SARS-CoV-2 at Both Protein and RNA Level.
Result: Notably, SARS-CoV-2 R203K/G204R polymorphisms modify the predicted binding of putative HLA-restricted T-cell epitopes containing these residues (Supplemental Table 2).
Result: Of the 455,774 circulating variants there were 29 amino acid polymorphisms present in >5% of the deposited sequences (of a total of 9413 sites; Supplemental Table 1) including the spike D614G variant (B.1 lineage) that emerged early in the pandemic and the adjacent R203K/G204R variants (B.1.1 lineage) in the nucleocapsid protein that formed one of the main variants emerging from Europe in early 2020.
Result: The adaptive potential of differential expression of sgRNAs is supported by a recent study by Thorne and colleagues that demonstrates that the B.1.1.7
Novel Nested-Seq Approach for SARS-CoV-2 Real-Time Epidemiology and In-Depth Mutational Profiling in Wastewater.
PMID: 34445204
2021
International journal of molecular sciences
Result: Although these mutations are located on the linker region (LKR) of the nucleocapsid phosphoprotein, which spans from position 175-254aa, only R203K belongs to the LKR's crucial Ser/Arg (SR)-rich motif that contains putative phosphorylation sites.
Result: Five well-characterized missense mutations, D614G (23403A>G)-S gene, Q57H (25563G>T)-ORF3a gene, P323L (14408C>T):ORF1ab/RdRP gene, R203K (28881G>A):N gene, and
Emergence and spread of the potential variant of interest (VOI) B.1.1.519 of SARS-CoV-2 predominantly present in Mexico.
Dynamics of SARS-CoV-2 mutations reveals regional-specificity and similar trends of N501 and high-frequency mutation N501Y in different levels of control measures.
Method: For instance, if 1 week presented 30% of genomes with the mutation R203K, and the number of cases on Monday of that week was 100.
Method: In the case of MF mutation R203K, R203, and N501, we multiply the relative frequencies of the genomes with the state of interest (R203K, R203 or N501) in a determined week by the number of cases in the day.
Result: This explains why this mutation did not increases its frequency steadily and can be an evidence of constant competition between MFR203K and not-MFR203K.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Frontiers in immunology
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