Result: The earlier R203K/G204R was shown experimentally to enhance the ability of N-protein to form condensates, and R203M - prevalent in the Delta variant - was recently reported to enhance viral replication.
Discussion: Earlier examinations of emerging mutations in N proteins, going back to June 2020, were necessarily more limited in scope, and while sufficient to examine hot spots and identify key replacements such as R203K/G204R, it was not yet possible to draw conclusions from a survey of the entire mutational landscape.
Phylogeography and genomic epidemiology of SARS-CoV-2 in Italy and Europe with newly characterized Italian genomes between February-June 2020.
The dynamics of circulating SARS-CoV-2 lineages in Bogor and surrounding areas reflect variant shifting during the first and second waves of COVID-19 in Indonesia.
Result: Unique changes were found in Indonesian lineages belonging to B.1.1.398 (N_G204R and N_R203K), B.1.470 (NSP_12_P227L) and B.1.466.2 (S_N439K and NSP6_L75F) lineages.
Unusual N Gene Dropout and Ct Value Shift in Commercial Multiplex PCR Assays Caused by Mutated SARS-CoV-2 Strain.
Discussion: According to the same tool, the frequency of the four amino acid substitutions, R203K, G204R, A208G and M234I, in 9.525.430 sequences available from the GISAID database (as of 29 March 2022, with collection days 2020-1-29, 2020-1-29, 2020-3-3, 2020-1-26 to 2022-3-29) was 44%, 43%, <0.5% and 2%, respectively.
Discussion: Sequencing analysis revealed four amino acid mutations (R203K, G204R, A208G, M234I) in the N protein sequence of all our B.1.1.318 isolates.
Discussion: The R203K and G204R amino acid changes have been found in several variants beside B.1.1.318 and are considered to contr
Isolation and Genomic Characterization of SARS-CoV-2 Omicron Variant Obtained from Human Clinical Specimens.
Introduction: Omicron has posed a serious public health concern due to the mutations/deletions associated with increased binding affinity to ACE2 (S:Q498R and S:N501Y), increased transmissibility (S:H655Y, S:N679K, and S:P681H), increased viral load (N:R203K and N:G204R), innate immune evasion (ORF1a:L3674-, ORF1a:S3675-, and ORF1a:G3676), and S-gene target failure (S:H69-).
Clinico-Genomic Analysis Reiterates Mild Symptoms Post-vaccination Breakthrough: Should We Focus on Low-Frequency Mutations?
Discussion: Moreover, the mutation R203K/G204R was found to display a different protein structure morphology from the control and an enhanced intraviral interaction of R203K/G204R in the N and E proteins in molecular docking and protein structure prediction analysis.
Identification of a novel SARS-CoV-2 variant with a truncated protein in ORF8 gene by next generation sequencing.
Result: These changes included, but were not limited to, D614G in Spike; R203K and G204K in N; and an out-of-frame deletion of five nucleotides in ORF7A, leading to its premature truncation.
A Detailed Overview of Immune Escape, Antibody Escape, Partial Vaccine Escape of SARS-CoV-2 and Their Emerging Variants With Escape Mutations.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Frontiers in cellular and infection microbiology
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