Novel Nested-Seq Approach for SARS-CoV-2 Real-Time Epidemiology and In-Depth Mutational Profiling in Wastewater.
PMID: 34445204
2021
International journal of molecular sciences
Result: Five well-characterized missense mutations, D614G (23403A>G)-S gene, Q57H (25563G>T)-ORF3a gene, P323L (14408C>T):ORF1ab/RdRP gene, R203K (28881G>A):N gene, and G204R (28883G>C):N gene, were initially targeted as proof-of-principle of our methodology to perform the analysis and quantification of SARS-CoV-2 mutations/strains in wastewater samples.
Result: Interestingly, the PMID: 34452390
2021
Viruses
Discussion: This included T85I in the nsp2 gene, P423L in the nsp12 gene, D614G in the S gene, and Q57H in the ORF3a gene.
Molecular characterization of SARS-CoV-2 from Bangladesh: implications in genetic diversity, possible origin of the virus, and functional significance of the mutations.
Discussion: One study mentioned that the Q57H mutation in ORF3a protein may decrease ion permeability by creating a tighter constriction in channel pore and possibly decrease viral release and immune response (Ul Alam et al.).
Discussion: Our analysis suggested the Q57H decreases protein stability with altered protein function may result in loss of a CD4+ T Cell epitope similar to other studies.
Discussion: There two other functionally significant mutations which are Q57H in ORF3a and S194L in N protein.
Higher entropy observed in SARS-CoV-2 genomes from the first COVID-19 wave in Pakistan.
Result: The most frequent variants were observed at positions 5' UTR; 241C>T (78.8%), S gene D614G (76%) Orf1ab; Nsp3 924F (74%), Orf1ab; RdRp P4715L (72%), Orf3a&nbs
Discussion: The most common non-synonymous variants observed were S D614G, Orf1ab RdRp gene P4715L and, Orf3a Q57H which have previously been reported from Europe and the United States.
Evolution, Mode of Transmission, and Mutational Landscape of Newly Emerging SARS-CoV-2 Variants.
Genome-wide identification and prediction of SARS-CoV-2 mutations show an abundance of variants: Integrated study of bioinformatics and deep neural learning.
PMID: 34812411
2021
Informatics in medicine unlocked
Result: Mutations [L5F, and R80I], S97I, and [L46C, V48G, G49I, Q57H, W131R, G172V, Q185H, and Y206S] were identified to have a deleterious effect on ORF7a, ORF8, and ORF3a proteins, respectively.
Result: Out of 46, 25 missense mutations (D1118H, S194L, R262H, M809L, P314L, A8D, PMID: 34845452
2021
bioRxiv
Abstract: To further characterize the mechanism, we tested a natural ORF3a Beta variant, Q57H, and a mutant with deletion of the highly conserved residue, DeltaG188.
Method: The ORF3a mutant variants (Q57H and DeltaG188) were generated by overlapping PCR with mutant-specific primers (Table S1) and cloned onto the same pLVX-EF1alpha-IRES-Puro plasmid via Gibson assembly method.
Discussion: For example, the Q57H mutant was predicted to bind S protein, whereas the wildtype does not.
Discussion: However, our data cannot rule out the possibility that the Q57H mutant may have other effects on viral pathogenesis.
Discussion: It would be of interest to test whether the
A next generation sequencing (NGS) analysis to reveal genomic and proteomic mutation landscapes of SARS-CoV-2 in South Asia.
PMID: 34841355
2021
Current research in microbial sciences
Result: According to the H-clades (Q57H), 71 South Asian samples (13.71%) were clustered where the samples of India (44) were found as the most dominant one.
Table: Q57H
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021.
SARS_CoV_2 mutation literature information.
PMID: 
2021
International journal of molecular sciences
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