D155Y substitution of SARS-CoV-2 ORF3a weakens binding with Caveolin-1.
PMID: 35126886
2022
Computational and structural biotechnology journal
Introduction: G251V and Q57H exhibit severe virulence property.Viral infection consists of several steps starting from viral entry, intracellular trafficking, replication, assembly and then release of the viral particles.
Introduction: The mutation patterns of ORF3a gene have been characterized as largely non-synonymous and increasingly deleterious (Q57H, H93Y, R126T, L127I, W128L, L129F, W131C, D155Y, D173Y, G196V, and G251V).
Table: Q57H
Discussion: <
Genome-Wide Characterization of SARS-CoV-2 Cytopathogenic Proteins in the Search of Antiviral Targets.
Abstract: To further characterize the mechanism, we tested a natural ORF3a Beta variant, Q57H, and a mutant with deletion of the highly conserved residue, DeltaG188.
Method: The ORF3a mutant variants (Q57H and DeltaG188) were generated by overlapping PCR with mutant-specific primers (see Table S1 in the supplemental material) and cloned onto the same pLVX-EF1alpha-IRES-Puro plasmid via the Gibson assembly method.
Discussion: For example, the Q57H mutant was predicted to bind the S protein, whereas the wild type does not.
Discussion: However, our data cannot rule out the possibility that the Q57H mutant may have other effects on viral pathogenesis.
Discussion: It would be of interest to test whether the
SARS-CoV-2 Mutations and Their Impact on Diagnostics, Therapeutics and Vaccines.
Introduction: In the US, Q57H mutation in the ORF3a region, and S24L and L84S in ORF8 was found to be prevalent, suggesting a positive effect on transmission or virulence.
Result: Various mutations were present in the stomach lining biopsy, especially the Q57H (ORF3a), reaching 98.6%, but were not detected in other tissues.
SARS-CoV-2 Mutations and COVID-19 Clinical Outcome: Mutation Global Frequency Dynamics and Structural Modulation Hold the Key.
PMID: 35386683
2022
Frontiers in cellular and infection microbiology
Discussion: A study by reported that the mutation Q57H, which is observed in our mortality patients, is consistently present in high frequency at all the time points.
Discussion: Another study describes the loss of function of orf3b protein, an accessory protein, due to the truncation of orf3a protein by Q57H mutation.
Discussion: Intriguingly, 5/26 significant mutations (C241T, Q57H, F924F, P4715L, D614G) in the mortality patients did not exhibit any frequency flip with a high frequency occurrence observed in the cohort as well as the global level.
In-silico genomic landscape characterization and evolution of SARS-CoV-2 variants isolated in India shows significant drift with high frequency of mutations.
PMID: 35233173
2022
Saudi journal of biological sciences
Table: Q57H
A Detailed Overview of Immune Escape, Antibody Escape, Partial Vaccine Escape of SARS-CoV-2 and Their Emerging Variants With Escape Mutations.
Introduction: evaluated the mutations in the ORF3 region and found the five most frequent mutations, which are Q57H, Q57H + A99V, V13L, G252V, and G196V.
Introduction: found that the Q57H mutation (25563G>T) is located in the variants in the USA in high frequency.
Table: Q57H
Genomic Diversity of SARS-CoV-2 in Algeria and North African Countries: What We Know So Far and What We Expect?
Result: In addition, mutations affecting other protein sequences appeared frequently including; N:RG203KR and N:G212V (Nucleocapsid protein N) with (636 genomes, 39.01%) and (339 genomes, 20.79%), respectively, M:I82T (Membrane protein) (411 genomes, 25, 25%), NSP3:T428I (phosphoesterase, papain-like proteinase) (400, genomes, 24.53%), ORF3a protein (ORF3a:Q57H) (350 genomes, 21, 47%), S:N501Y (Spike protein) (163 genomes, 10.0%), and E
Worldwide SARS-CoV-2 haplotype distribution in early pandemic.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Microbiology resource announcements
Browser Board
Co-occurred Entities
Filtrator
ViMRT