Isolation of SARS-CoV-2 B.1.1.28.2 (P2) variant and pathogenicity comparison with D614G variant in hamster model.
PMID: 34959053
2022
Journal of infection and public health
Introduction: This variant has about 17 mutations, including N501Y, P681H, 69-70 deletion; the ORF8 Q27stop mutation outside the spike protein and is adapted to be more transmissible.
Identification of a novel SARS-CoV-2 variant with a truncated protein in ORF8 gene by next generation sequencing.
Result: Comparison of this novel variant with genome signature of B.1.1.291 indicates that this novel variant contains two unique mutations (ORF8 Q27stop, NSP15 V127F) that other B.1.1.291 variants do not have, while lack of some other mutations (ORF1ab F106F, ORF1ab Y831Y, ORF1ab N73N, noncoding C29784T, and noncoding C241T) in Pango Lineage B.1.1.291 (Table 1).
Result: Interestingly, the only nonsynonymous mutation in this novel variant different from other Pango Lineage B.1.1.291 is the ORF8 Q27stop
An issue of concern: unique truncated ORF8 protein variants of SARS-CoV-2.
Abstract: Among many contributing mutations, Q27STOP, a mutation in the ORF8 protein, defines the B.1.1.7 lineage of SARS-CoV-2, engendering the second wave of COVID-19.
Abstract: In the pr
Discussion: The Q27STOP mutation in the ORF8 protein has been discovered to cause 47 distinct truncated ORF8 variations.
Discussion: The appearance of the premature stop codon in the ORF8 gene encoding the NS8 (ORF8) protein (Q27stop) not only generates the truncated form of this protein due to the deletion of almost 80% of the ORF8 protein, but also eliminates the whole immunopeptidome associated with this part of protein.
Genetic Characteristics and Phylogeny of 969-bp S Gene Sequence of SARS-CoV-2 from Hawaii Reveals the Worldwide Emerging P681H Mutation.
Discussion: The Orf8 mutations are Q27stop, R52I, and Y73C.
SARS-CoV-2 variants combining spike mutations and the absence of ORF8 may be more transmissible and require close monitoring.
PMID: 33676232
2021
Biochemical and biophysical research communications
Abstract: However, VOC-202012/01 has also a mutation (Q27stop) that truncates the ORF8, a likely immune evasion protein.
Result: The
Result: The Q27stop is present in the VOC-202012/01 variant, which lacks the widespread D614G mutation.
Result: Variants with Q27stop and E106stop did not form any clear cluster, despite being frequent in our dataset.
Result: When considered independently, the most frequent ORF8 nonsense mutations were Q18stop (n = 534), E64stop (n = 348), E110stop (n = 306), Q27stop (n = 158) and E106stop (n = 123) (Table 1).
Structure-Function Analyses of New SARS-CoV-2 Variants B.1.1.7, B.1.351 and B.1.1.28.1: Clinical, Diagnostic, Therapeutic and Public Health Implications.
Conclusion: In the 501Y.V1 variant, we hypothesized that R52I mutation could affect multimer assembly of the ORF8 protein, however another Q27Stop deletion apparently showed no effect on viral transcription.
SARS-CoV-2 gene content and COVID-19 mutation impact by comparing 44 Sarbecovirus genomes.
Result: For example, the B.1.1.7 lineage includes mutations C5388A (orf1ab:A1708D) in a string of 7 perfectly conserved amino acids in a well-conserved region of nsp3, C14676T, a synonymous change in a large SCE in RdRp (situated between two conserved structures predicted by RNAz so possibly part of a containing structure too large for the prediction algorithm), T24506G (spike:S982A) in an extremely well-conserved region of S2, a three-nucleotide mutation at position 28280 (nucleocapsid:D3L) which weakens the initiation context of ORF9b, and C27972T (
Recent progress on the mutations of SARS-CoV-2 spike protein and suggestions for prevention and controlling of the pandemic.
PMID: 34146731
2021
Infection, genetics and evolution
Introduction: In addition to Spike mutations, the ORF8 Q27stop mutation truncates the ORF8 protein and thus allows other downstream mutations to accumulate.
Introduction: This suggests a potential role of the Q27stop mutation in compensating for the reduction in vaccine or antibody activity, probably caused by certain mutations of the 20I/501Y.V1 variant.
A Novel SARS-CoV-2 Viral Sequence Bioinformatic Pipeline Has Found Genetic Evidence That the Viral 3' Untranslated Region (UTR) Is Evolving and Generating Increased Viral Diversity.
Abstract: Almost all VOC-containing genomes possess one stop codon in ORF8 gene ( Result: Regardless the fact that all genomes from B.1.1.7 lineage contain the Q27* variant, around 14% of these genomes contain another downstream stop codon, Q68* (Figure 3E), confirming that ORF8 is prone to accumulate non-sense variants and B.1.1.7 lineage transmits successfully without expression of ORF8.
Result: The emergent SARS-CoV-2 B.1.1.7 lineage in United Kingdom contained at least 21 non-synonymous substitutions including a stop codon in the ORF8 gene (Q27*, Figure 3E) and is constantly increasing its worldwide viral frequency in GISAID database since the beginning of November 30, 2020 until the time of writing of this manuscript.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Journal of infection and public health
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