Result: Binding level results of
T29I,
V367F,
A706V, and
A831V demonstrated that these substitutions had low binding affinity in HLA-A01:01, HLA-B07:02, and HLA-B35:01 compared to the wild type, while
H49Y,
Q239K,
V483A,
D839Y,
S943P,
A1078S, and
P1263L substitutions still had strong binding affinity with HLA molecules.