Real-Time RT-PCR Allelic Discrimination Assay for Detection of N501Y Mutation in the Spike Protein of SARS-CoV-2 Associated with B.1.1.7 Variant of Concern.
Result: Whereas, AU.2 carries a mutation at positions N439K, P681R and G1251V.
Discussion: We observed that AU.2 is closely related to B.1.4662 (Indonesian lineage), as both lineages carry the same amino acid mutations N439K in RBD and P681R in non RBD regions of spike protein.
Rapidly Identifying New Coronavirus Mutations of Potential Concern in the Omicron Variant Using an Unsupervised Learning Strategy.
Introduction: For example, we observed the emergence of the P681R mutation in the U.S.
Introduction: The Omicron variants thus far display the P681H mutation observed in earlier Alpha variants, unlike the P681R mutation observed in Delta, which has been documented experimentally to contribute to increased infectivity.
SARS-CoV-2 Beta and Delta variants trigger Fc effector function with increased cross-reactivity.
Method: The SARS-CoV-2 Wuhan-1 spike, cloned into pCDNA3.1 was mutated using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) and NEBuilder HiFi DNA Assembly Master Mix (NEB) to include D614G (original) or lineage defining mutations for Alpha (DEL69-70, DEL144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), Beta (L18F, D80A, D215G, 242-244del, K417N, E484K, N501Y, D614G and A701V<
A Detailed Overview of Immune Escape, Antibody Escape, Partial Vaccine Escape of SARS-CoV-2 and Their Emerging Variants With Escape Mutations.
Introduction: Few mutations include K417T/N, E484K, L452R, N501Y, P681R, D614G, etc.
Introduction: In this direction, we illustrated the mutations in the RBD region (N439K, L452R, E484K, N501Y, K444R) and other regions (D614G, P681R) of the S-glycoprotein.
Introduction: Several mutations have been observed among VOI, and so
Table: P681R
Rapid detection of the widely circulating B.1.617.2 (Delta) SARS-CoV-2 variant.
Abstract: Whole genome sequencing of 46 randomly selected samples validated the strains identified as positive and negative for the B.1.617.2 (Delta) variant and confirmed the S gene deletion in addition to B.1.617.2 characteristic mutations
Result: Genome sequencing of 46 samples confirmed with 100% accuracy the S gene deletion in addition to B.1.617.2 characteristic L452R, T478K, P681R, and D950N mutations located in the spike protein.
Result: In addition, non-synonymous mutations C>G (T19R), T>G (L452R), C>A (T478K), C>G (P681R) and G>A G (D950N) were identified.
High throughput sequencing based direct detection of SARS-CoV-2 fragments in wastewater of Pune, West India.
PMID: 34688738
2022
The Science of the total environment
Result: Notably, nine mutations in the Spike region (S: L452R, S:C480R, S: E484Q, S: D614G, S: P681R, S: N801, S: D950N, S: Q1071H, S: P1140) were observed in this study (Table 1, Supplementary Table 2).
Table: P681R
Spread of Gamma (P.1) Sub-Lineages Carrying Spike Mutations Close to the Furin Cleavage Site and Deletions in the N-Terminal Domain Drives Ongoing Transmission of SARS-CoV-2 in Amazonas, Brazil.
4Method: The variants N679K, P681H and P681R loops were built by using Point mutant (""pmut"") scan application of Rosetta onto a modeled native loop."
Method: Modeling the relative binding strength of N679K, P681H and P681R variants to furin.
Discussion: Several P.1 Brazilian su
Discussion: The major P.1+ lineages circulating outside the Amazonas state were P.1.7 (P.1+P681H), which probably arose in the state of Sao Paulo around mid-February 2021 and is currently detected in at least 10 different states from all country regions; and P.1.8 (P.1+P681R), that probably arose in the state of Rio de Janeiro in early May 2021 and spread to the Southern (Santa Catarina) and Northern (Amazonas) regions.
Emergency SARS-CoV-2 Variants of Concern: Novel Multiplex Real-Time RT-PCR Assay for Rapid Detection and Surveillance.
Abstract: This multiplex PCR typing method was established to detect 9 mutations with specific primers and probes (DeltaHV 69/70, K417T, K417N, L452R, E484K, E484Q, N501Y, P681H, and P681R) against the receptor-binding domain of the spike protein of SARS-CoV
Result: Using the LightCycler 96 Thermocycler (Roche, Mannheim, Germany), the LoD obtained from the 20 replicate tests was 30 copies/muL for the mutant N501Y and 60 copies/muL for the mutants DeltaHV 69/70, K417N, E484K, L452R, and P681R (Table 1).
Divergent SARS-CoV-2 Omicron-reactive T and B cell responses in COVID-19 vaccine recipients.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Microbiology spectrum
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