Real-Time RT-PCR Allelic Discrimination Assay for Detection of N501Y Mutation in the Spike Protein of SARS-CoV-2 Associated with B.1.1.7 Variant of Concern.
Result: Whereas, AU.2 carries a mutation at positions N439K, P681R and G1251V.
Discussion: We observed that AU.2 is closely related to B.1.4662 (Indonesian lineage), as both lineages carry the same amino acid mutations N439K in RBD and P681R in non RBD regions of spike protein.
Rapidly Identifying New Coronavirus Mutations of Potential Concern in the Omicron Variant Using an Unsupervised Learning Strategy.
Introduction: For example, we observed the emergence of the P681R mutation in the U.S.
Introduction: The Omicron variants thus far display the P681H mutation observed in earlier Alpha variants, unlike the P681R mutation observed in Delta, which has been documented experimentally to contribute to increased infectivity.
SARS-CoV-2 Beta and Delta variants trigger Fc effector function with increased cross-reactivity.
Method: The SARS-CoV-2 Wuhan-1 spike, cloned into pCDNA3.1 was mutated using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) and NEBuilder HiFi DNA Assembly Master Mix (NEB) to include D614G (original) or lineage defining mutations for Alpha (DEL69-70, DEL144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), Beta (L18F, D80A, D215G, 242-244del, K417N, E484K, N501Y, D614G and A701V<
A Detailed Overview of Immune Escape, Antibody Escape, Partial Vaccine Escape of SARS-CoV-2 and Their Emerging Variants With Escape Mutations.
Introduction: Few mutations include K417T/N, E484K, L452R, N501Y, P681R, D614G, etc.
Introduction: In this direction, we illustrated the mutations in the RBD region (N439K, L452R, E484K, N501Y, K444R) and other regions (D614G, P681R) of the S-glycoprotein.
Introduction: Several mutations have been observed among VOI, and so
Table: P681R
Rapid detection of the widely circulating B.1.617.2 (Delta) SARS-CoV-2 variant.
Abstract: Whole genome sequencing of 46 randomly selected samples validated the strains identified as positive and negative for the B.1.617.2 (Delta) variant and confirmed the S gene deletion in addition to B.1.617.2 characteristic mutations including L452R, T478K, P681R and D950N located in the spike protein.
Result: Genome sequencing of 46 samples confirmed with 100% accuracy the S gene deletion in addition to B.1.617.2 characteristic L452R, T478K, P681R, and D950N mutations located in the spike protein.
Result: In addition, non-synonymous mutations C>G ( PMID: 34688738
2022
The Science of the total environment
Result: Notably, nine mutations in the Spike region (S: L452R, S:C480R, S: E484Q, S: D614G, S: P681R, S: N801, S: D950N, S: Q1071H, S: P1140) were observed in this study (Table 1, Supplementary Table 2).
Table: P681R
Spread of Gamma (P.1) Sub-Lineages Carrying Spike Mutations Close to the Furin Cleavage Site and Deletions in the N-Terminal Domain Drives Ongoing Transmission of SARS-CoV-2 in Amazonas, Brazil.
4Method: The variants N679K, P681H and P681R loops were built by using Point mutant (""pmut"") scan application of Rosetta onto a modeled native loop."
Method: Modeling the relative binding strength of N679K, P681H and P681R variants to furin.
Figure: Arrows indicate the native position of variant mutations N679K and P681H/R.
Discussion: A previous study conducted by our group detected the concurrent emergence of multiple P.1+ lineages bearing NTD deletions (including Delta144 and Delta141-44) in several Brazilian states and the present molecular survey identified the emergence of P.1 lineages with mutations S:N679K and
Emergency SARS-CoV-2 Variants of Concern: Novel Multiplex Real-Time RT-PCR Assay for Rapid Detection and Surveillance.
Result: Using the LightCycler 96 Thermocycler (Roche, Mannheim, Germany), the LoD obtained from the 20 replicate tests was 30 copies/muL for the mutant N501Y and 60 copies/muL for the mutants DeltaHV 69/70, K417N, E484K, L452R, and P681R (Table 1).
Table: P681R
Figure: (A) VOI (K417T and P681H) and VOC (DeltaHV69-79, K417N, L452R, E484K, E484Q, N501Y, and P681R).
Figure: Yellow, DeltaHV69-70; light violet, K417N and 417T; green,
Divergent SARS-CoV-2 Omicron-reactive T and B cell responses in COVID-19 vaccine recipients.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Microbiology spectrum
Browser Board
Co-occurred Entities
Filtrator
ViMRT