literature

SARS_CoV_2 mutation literature information.

SARS-CoV-2 Beta and Delta variants trigger Fc effector function with increased cross-reactivity.

PMID: 35233544 2022 Cell reports. Medicine

Method: The SARS-CoV-2 Wuhan-1 spike, cloned into pCDNA3.1 was mutated using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) and NEBuilder HiFi DNA Assembly Master Mix (NEB) to include D614G (original) or lineage defining mutations for Alpha (DEL69-70, DEL144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), Beta (L18F, D80A, D215G, 242-244del, K417N, E484K, N501Y,

Table: P681H

Rapidly Identifying New Coronavirus Mutations of Potential Concern in the Omicron Variant Using an Unsupervised Learning Strategy.

PMID: 35233566 2022 Research square

Abstract: To build an investigative framework, we have applied an unsupervised machine learning approach to 4296 Omicron viral genomes collected and deposited to GISAID as of December 14, 2021, and have identified a core haplotype of 28 polymutants (A67V, T95I, G339D, R346K, S371L, S373P, Introduction: Finally, there are two mutations (N679K, P681H) in the S1/S2 cleavage segment.

Introduction: The Omicron variants thus far display the P681H mutation observed in earlier Alpha variants, unlike the P681R mutation observed in Delta, which has been documented experimentally to contribute to increased infectivity.

Design of SARS-CoV-2 Variant-Specific PCR Assays Considering Regional and Temporal Characteristics.

PMID: 35285246 2022 Applied and environmental microbiology

Result: As a result, for the Alpha variant, we identified nine mutations in the spike gene: S:Delta69/70, S:Delta144, S:N510Y, S:A570D, S:D614G, S:P681H, S:T716I, S:S982A, and S:D1118H.

Result: To further confirm whether the RT-qPCR results were correct, we conducted NGS analysis to examine eight mutation markers for the Alpha variant (S:Delta69/70,

A screening strategy for identifying the dominant variant of SARS-COV-2 in the fifth peak of Kurdistan- Iran population using HRM and Probe-based RT-PCR assay.

PMID: 35271889 2022 Journal of virological methods

Table: P681H

Evolutionary history of the SARS-CoV-2 Gamma variant of concern (P.1): a perfect storm.

PMID: 35266951 2022 Genetics and molecular biology

Introduction: Nine key mutations involving the S glycoprotein have been identified: 69/70del, 144del, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H.

A SARS-CoV-2 Wuhan spike virosome vaccine induces superior neutralization breadth compared to one using the Beta spike.

PMID: 35273217 2022 Scientific reports

Method: Pre-fusion spike protein ectodomain DNA constructs were designed containing the following mutations compared to the Wuhan variant (Wuhan Hu-1; GenBank: MN908947.3): deletion of H69, V70 and Y144, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H in Alpha; L18F, D80A, D215G, L242H, R246I, K417N, E484K, N501Y, D614G and A701V in Beta;

Omicron variant (B.1.1.529) of SARS-CoV-2: understanding mutations in the genome, S-glycoprotein, and antibody-binding regions.

PMID: 35258772 2022 GeroScience

Abstract: We also evaluated mutations in the antibody-binding regions and observed some important mutations overlapping those of previous variants including N501Y, D614G, H655Y, N679K, and P681H.

Introduction: Some mutations including T478K, N501Y, N655Y, N679K, and P681H overlap with

Result: Mutations previously reported as important in different variants were N501Y, D614G, H655Y, N679K, and P681H.

Evolution of the SARS-CoV-2 spike protein in the human host.

PMID: 35246509 2022 Nature communications

Result: A similar observation has been made in studies of spike material isolated directly from the Alpha variant virus and the same P681H substitution has also been observed in the recent B.1.1.529 (Omicron) variant spike.

Result: Our data suggest that this substitution P681R in Kappa and Delta spikes, as well as P681H in Omicron spike, will also increase stability of the receptor-bound form of these spikes, accounting at least in part for their increased transmissibility.

Result: This observation is consistent with one of the changes in the Alpha spike being the substitution P681H, which generates an even more basic furin-cleavage site (

Emergence and Spread of a B.1.1.28-Derived P.6 Lineage with Q675H and Q677H Spike Mutations in Uruguay.

PMID: 34578382 2021 Viruses

Introduction: The lineage P.3 (former VOI Theta) emerged in the Philippines, and it includes substitutions S:E484K/N501Y/P681H among the lineage-defining mutations; the first sample was collected on 8 January 2021, and later it further spread to the USA, Germany, and Malaysia, among other countries.

Discussion: Moreover, mutations close to or at the polybasic cleavage site at the S1/S2 boundary have been reported in several VOCs and VOIs, including: Alpha (S:P681H), Beta (A701V), Delta (P681R), Eta (Q677H), Iota (A701V), Kappa (P681R), and Theta (P.3, P681H).

Neurological pathophysiology of SARS-CoV-2 and pandemic potential RNA viruses: a comparative analysis.

PMID: 34757622 2021 FEBS letters

Abstract: We present new insight into key mutations in SARS-CoV-2 variants B.1.1.7 (P681H) and B.1.617.2 (P681R), which may impact on neuropilin 1 (NRP1) binding and CNS invasion.

Browser Board

Co-occurred Entities

Filtrator