Discussion: P681H resides in the furin cleavage site in this strain predictably with improved function.
Discussion: However, did not find any evidence of a particular strain of SARS-CoV2 that has over-infecting ability in the population although convincingly showed the widespread infectivity of this strain in southern USA population,.However, very recently a super-infective b.1.1.7 strain carries a set of spike protein mutations including D614G, N501Y and P681H.
Discussion: We also detected P681H in a strain (isolated in New Mexico, USA, in September isolate,) in several people (e.g., Acc no.
SARS-CoV-2 Beta and Delta variants trigger Fc effector function with increased cross-reactivity.
Method: The SARS-CoV-2 Wuhan-1 spike, cloned into pCDNA3.1 was mutated using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) and NEBuilder HiFi DNA Assembly Master Mix (NEB) to include D614G (original) or lineage defining mutations for Alpha (DEL69-70, DEL144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), Beta (L18F, D80A, D215G, 242-244del, K417N, E484K, N501Y,
Table: P681H
Rapid detection of the widely circulating B.1.617.2 (Delta) SARS-CoV-2 variant.
Discussion: Previous in vitro studies already showed the association of some of these mutations with increased infectivity, ACE2 interaction (N501Y, P681H), or immune evasion (K417N, N440K, G446S, S477N, E484A/K, Q493R).
Design of SARS-CoV-2 Variant-Specific PCR Assays Considering Regional and Temporal Characteristics.
PMID: 35285246
2022
Applied and environmental microbiology
Result: As a result, for the Alpha variant, we identified nine mutations in the spike gene: S:Delta69/70, S:Delta144, S:N510Y, S:A570D, S:D614G, S:P681H, S:T716I, S:S982A, and S:D1118H.
Result: To further confirm whether the RT-qPCR results were correct, we conducted NGS analysis to examine eight mutation markers for the Alpha variant (S:Delta69/70,
Emergence in southern France of a new SARS-CoV-2 variant harbouring both N501Y and E484K substitutions in the spike protein.
Introduction: In particular, P681H is located in the cleavage site of the S1-S2 subunits of the spike and is observed in other variants, including the recently emerging Omicron variant.
Introduction: These include the substitutions N501Y and E484K, which are present in the Beta, Gamma, Theta, and Omicron variants, F490S, which is present in the Lambda variant, and P681H, which is present in the Lambda and Omicron variants.
Table: P681H
Spread of Gamma (P.1) Sub-Lineages Carrying Spike Mutations Close to the Furin Cleavage Site and Deletions in the N-Terminal Domain Drives Ongoing Transmission of SARS-CoV-2 in Amazonas, Brazil.
Abstract: As lineages P.1.4 (S:N679K) and P.1.6 (S:P681H) expanded (Re > 1) from March to July 2021, the lineage P.1 declined (Re < 1) and the median Ct value of SARS-CoV-2 positive cases in Amazonas significantly decreases.
Abstract: Our study reveals a sharp increase in the relative prevalence of Gamma plus (P.1+) variants, designated Pango Lineages P.1.3 to P.1.6, harboring two types of additional Spike changes: deletions in the N-terminal (NTD) domain (particularly Delta144 or Delta141-144) associated with resistance to anti-NTD neutralizing antibodies or mutations at the S1/S2 junction (N679K or P681H) that probably enhance the binding affinity to the furin cleavage site, as suggested by our molecular dynamics s
Emergency SARS-CoV-2 Variants of Concern: Novel Multiplex Real-Time RT-PCR Assay for Rapid Detection and Surveillance.
Method: To verify the assay accuracy in our RT-PCR method, VOCs were also detected used VirSNiP SARS-CoV-2 mutation assays for strain surveillance (TIB Molbiol, Berlin, Germany), which used real-time RT-PCR postmelting curve analysis to detect mutations targeting specific spike protein variations (HV69/70, K417N, L452R, E484K, N501Y, P681H, and P681R).
Figure:
Figure: (A) VOI (K417T and P681H) and VOC (DeltaHV69-79, K417N, L452R, E484K, E484Q, N501Y, and P681R).
Real-Time RT-PCR Allelic Discrimination Assay for Detection of N501Y Mutation in the Spike Protein of SARS-CoV-2 Associated with B.1.1.7 Variant of Concern.
Introduction: The VOC first detected in the United Kingdom represents the B.1.1.7 lineage (also known as 20I/501Y.V1, VOC202012/010, and Alpha) and contains three key mutations in the spike (S) gene: 69 to 70 deletion, N501Y, and P681H.
Rapidly Identifying New Coronavirus Mutations of Potential Concern in the Omicron Variant Using an Unsupervised Learning Strategy.
Abstract: To build an investigative framework, we have applied an unsupervised machine learning approach to 4296 Omicron viral genomes collected and deposited to GISAID as of December 14, 2021, and have identified a core haplotype of 28 polymutants (A67V, T95I, G339D, R346K, S371L, S373P, Introduction: Finally, there are two mutations (N679K, P681H) in the S1/S2 cleavage segment.
Introduction: The Omicron variants thus far display the P681H mutation observed in earlier Alpha variants, unlike the P681R mutation observed in Delta, which has been documented experimentally to contribute to increased infectivity.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Virus research
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