Abstract: This multiplex PCR typing method was established to detect 9 mutations with specific primers and probes (DeltaHV 69/70, K417T, K417N, L452R, E484K, E484Q, <
Method: To verify the assay accuracy in our RT-PCR method, VOCs were also detected used VirSNiP SARS-CoV-2 mutation assays for strain surveillance (TIB Molbiol, Berlin, Germany), which used real-time RT-PCR postmelting curve analysis to detect mutations targeting specific spike protein variations (HV69/70, K417N, L452R, E484K, N501Y, P681H, and P681R).
Table: P681H
Classical and Next-Generation Vaccine Platforms to SARS-CoV-2: Biotechnological Strategies and Genomic Variants.
PMID: 35206580
2022
International journal of environmental research and public health
Introduction: Eight genomic variants map to the spike glycoprotein, including N501Y in the receptor-binding domain, deletion 69_70, and P681H in the furin cleavage site.
Genomic Diversity of SARS-CoV-2 in Algeria and North African Countries: What We Know So Far and What We Expect?
Result: The amino acid substitutions (D614G, D614G, D796Y, T547K, N856K, N679K, N969K, P681H, L981F) in the spike protein, P314L, A1892T, T492I, I189V and A1892T in the non-structural proteins (NSP3, NSP4, NPS6, and NSP12b) occurred in 100% of analyzed SARS-CoV-2 genomes.
Discussion: Comparative genomic analysis of SARS-CoV-2 genomes revealed multiple crucial mutations to the
SARS-CoV-2 Omicron Spike recognition by plasma from individuals receiving BNT162b2 mRNA vaccination with a 16-week interval between doses.
Discussion: Previous in vitro studies already showed the association of some of these mutations with increased infectivity, ACE2 interaction (N501Y, P681H), or immune evasion (K417N, N440K, G446S, S477N, E484A/K, Q493R).
Rapid detection of the widely circulating B.1.617.2 (Delta) SARS-CoV-2 variant.
Method: The SARS-CoV-2 Wuhan-1 spike, cloned into pCDNA3.1 was mutated using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) and NEBuilder HiFi DNA Assembly Master Mix (NEB) to include D614G (original) or lineage defining mutations for Alpha (DEL69-70, DEL144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), Beta (L18F, D80A, D215G, 242-244del, K417N, E484K, N501Y,
Table: P681H
Analysis of SARS-CoV-2 variants B.1.617: host tropism, proteolytic activation, cell-cell fusion, and neutralization sensitivity.
Introduction: A similar mutation, P681H, has been identified in B.1.1.7, which were reported to promote cleavage of the S protein precursor and affect O-glycosylation of the spike protein, but may not substantially impact viral entry or cell-cell spread.
SARS-CoV-2 Mutations and Their Impact on Diagnostics, Therapeutics and Vaccines.
Design of SARS-CoV-2 Variant-Specific PCR Assays Considering Regional and Temporal Characteristics.
PMID: 35285246
2022
Applied and environmental microbiology
Result: As a result, for the Alpha variant, we identified nine mutations in the spike gene: S:Delta69/70, S:Delta144, S:N510Y, S:A570D, S:D614G, S:P681H, S:T716I, S:S982A, and S:D1118H.
Result: To further confirm whether the RT-qPCR results were correct, we conducted NGS analysis to examine eight mutation markers for the Alpha variant (S:Delta69/70,
A SARS-CoV-2 Wuhan spike virosome vaccine induces superior neutralization breadth compared to one using the Beta spike.
Method: Pre-fusion spike protein ectodomain DNA constructs were designed containing the following mutations compared to the Wuhan variant (Wuhan Hu-1; GenBank: MN908947.3): deletion of H69, V70 and Y144, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H in Alpha; L18F, D80A, D215G, L242H, R246I, K417N, E484K, N501Y, D614G and A701V in Beta;
SARS_CoV_2 mutation literature information.
PMID: 
2022
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