Introduction: This linage also exhibited a change at the residue 681 (P681H), one of the four residues comprising the furin cleavage site located between S1 and S2 domains in the spike protein (Rambaut et al., 2020).
Introduction: Thus, P681H residue substitution could additionally enhance this interaction.
Introduction: This mutation cooccurs with several mutations, including missense mutations (A570D, P681H, T716I, S982A, and D1118H), as well as disruptive in-frame deletions (H69-V70 and Y145).
Table: p.Pro681His
Combined RT-qPCR and pyrosequencing of a Spike glycoprotein polybasic cleavage motif can uncover pediatric SARS-CoV-2 infections associated with heterogeneous presentation.
PMID: 33893880
2021
Molecular and cellular pediatrics
Abstract: Moreover, due to the incremental transmissi
Result: Due to the incremental transmission of SARS-CoV-2 variants-of-concern, we note that the used strategy can uncover (Spike) P681H allowing the pre-selection of SARS-CoV-2 B.1.1.7 candidate specimens for deep sequencing.
Result: Remarkably, N501Y was seen in 2 other adult samples (Figure S7), but neither E484K nor P681H (data not shown).
Result: Using different sequencing primers (Table S1), we could not detect N501Y in the P681H sample, which is a key substitution for 3 variants-of-concern.
Result: Whereas we did not see the P681H substitution in any of the analyzed pediatric specimens, we could detect it in 1 adult (Figure S5).
Emerging variants of concern in SARS-CoV-2 membrane protein: a highly conserved target with potential pathological and therapeutic implications.
Result: The novel combination of M:I82T, the three signature Spike mutations (S:S494P, the S:P681H and S:T716I) from B.1.1.7, and the N:T205I mutation is therefore of particular concern.
Result: There were 10 other missense mutations present in at least 90% of the isolates in this clade and 8 of them were enriched by 73 to 146 fold compared to the general B.1 lineage including the 3 signature mutations in the spike protein (S:S494P, the S: Table: P681H
The Genetic Variant of SARS-CoV-2: would It Matter for Controlling the Devastating Pandemic?
PMID: 33907511
2021
International journal of biological sciences
Introduction: In addition, the P681H mutation has appeared many times independently and has become dominant in the local epidemic in Hawaii.
Introduction: The function of mutation of P681H is unclear but it locates near the furin-cleavage site, which is important for SARS-CoV-2 entry.
Introduction: Three mutations, namely N501Y, DeltaH69/DeltaV70 and P681H, locate in S protein.
Impact of the N501Y substitution of SARS-CoV-2 Spike on neutralizing monoclonal antibodies targeting diverse epitopes.
Introduction: There are seven substitutions (N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H) and three deletions (H69Del, V70Del, and Y144Del) in the spike of the N501Y.V1 variant comparing with the Wuhan-Hu-1 strain (wide type), with N501Y the only mutation in the ACE2 interface of the receptor binding domain (RBD).
The Impact on Infectivity and Neutralization Efficiency of SARS-CoV-2 Lineage B.1.351 Pseudovirus.
Introduction: In the case of B.1.1.7, a series of mutations in eight sites including D614G appeared in the spike protein: Delta69Delta70, Delta144Delta145, N501Y, A570D, P681H, T716I, S982A, and D1118H (; GISAID, accessed on 1 December 2020).
Surveillance of SARS-CoV-2 in Frankfurt am Main from October to December 2020 Reveals High Viral Diversity Including Spike Mutation N501Y in B.1.1.70 and B.1.1.7.
Introduction: Lineage B.1.1.7 has seventeen lineage-defining mutations including a deletion at positions 69/70 (Delta69/70) and 144, and substitutions N501Y and P681H in S.
Mutation Signatures and In Silico Docking of Novel SARS-CoV-2 Variants of Concern.
Result: In addition, the UK-VOC contains several nonsynonymous mutations that cause seven aa substitutions at positions N501Y, A570D, D614G, P681H, T716I, S982A, D1118H in S-protein, in which N501Y mutation occurs in the key residue of
Discussion: Along with the N501Y mutation in UK-VOC, it is speculated that the combination of the P681H mutation in the furin cleavage site and deletion of two amino acids at positions 69-70, which can alter the homology of the S-protein, is likely enhancing the transmissibility of SARS-CoV-2 and resulting in increased patient numbers.
SARS-CoV-2 mutations: the biological trackway towards viral fitness.
Introduction: P681H mutation lies near the furin cleavage site and might interfere with viral infectivity and transmission.
Introduction: VUI202012/01 had eight mutations in S protein of which N501Y, P681H, Delta69 and Delta70 have potential implications on viral infectivity.
SARS_CoV_2 mutation literature information.
PMID: 
2021
EXCLI journal
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