literature

SARS_CoV_2 mutation literature information.

SARS-CoV-2 Beta and Delta variants trigger Fc effector function with increased cross-reactivity.

PMID: 35233544 2022 Cell reports. Medicine

Method: The SARS-CoV-2 Wuhan-1 spike, cloned into pCDNA3.1 was mutated using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) and NEBuilder HiFi DNA Assembly Master Mix (NEB) to include D614G (original) or lineage defining mutations for Alpha (DEL69-70, DEL144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), Beta (L18F, D80A, D215G, 242-244del, K417N, E484K, N501Y,

Table: P681H

Evolutionary shift from purifying selection towards divergent selection of SARS-CoV2 favors its invasion into multiple human organs.

PMID: 35176330 2022 Virus research

Discussion: P681H resides in the furin cleavage site in this strain predictably with improved function.

Discussion: However, did not find any evidence of a particular strain of SARS-CoV2 that has over-infecting ability in the population although convincingly showed the widespread infectivity of this strain in southern USA population,.However, very recently a super-infective b.1.1.7 strain carries a set of spike protein mutations including D614G, N501Y and P681H.

Discussion: We also detected P681H in a strain (isolated in New Mexico, USA, in September isolate,) in several people (e.g., Acc no.

Omicron variant (B.1.1.529) of SARS-CoV-2: understanding mutations in the genome, S-glycoprotein, and antibody-binding regions.

PMID: 35258772 2022 GeroScience

Abstract: We also evaluated mutations in the antibody-binding regions and observed some important mutations overlapping those of previous variants including N501Y, D614G, H655Y, N679K, and P681H.

Introduction: Some mutations including T478K, N501Y, N655Y, N679K, and P681H overlap with

Result: Mutations previously reported as important in different variants were N501Y, D614G, H655Y, N679K, and P681H.

A screening strategy for identifying the dominant variant of SARS-COV-2 in the fifth peak of Kurdistan- Iran population using HRM and Probe-based RT-PCR assay.

PMID: 35271889 2022 Journal of virological methods

Table: P681H

A SARS-CoV-2 Wuhan spike virosome vaccine induces superior neutralization breadth compared to one using the Beta spike.

PMID: 35273217 2022 Scientific reports

Method: Pre-fusion spike protein ectodomain DNA constructs were designed containing the following mutations compared to the Wuhan variant (Wuhan Hu-1; GenBank: MN908947.3): deletion of H69, V70 and Y144, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H in Alpha; L18F, D80A, D215G, L242H, R246I, K417N, E484K, N501Y, D614G and A701V in Beta;

Evolutionary history of the SARS-CoV-2 Gamma variant of concern (P.1): a perfect storm.

PMID: 35266951 2022 Genetics and molecular biology

Introduction: Nine key mutations involving the S glycoprotein have been identified: 69/70del, 144del, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H.

SARS-CoV-2 Mutations and Their Impact on Diagnostics, Therapeutics and Vaccines.

PMID: 35273977 2022 Frontiers in medicine

Table: P681H

Evolution of the SARS-CoV-2 spike protein in the human host.

PMID: 35246509 2022 Nature communications

Result: A similar observation has been made in studies of spike material isolated directly from the Alpha variant virus and the same P681H substitution has also been observed in the recent B.1.1.529 (Omicron) variant spike.

Result: Our data suggest that this substitution P681R in Kappa and Delta spikes, as well as P681H in Omicron spike, will also increase stability of the receptor-bound form of these spikes, accounting at least in part for their increased transmissibility.

Result: This observation is consistent with one of the changes in the Alpha spike being the substitution P681H, which generates an even more basic furin-cleavage site (

Distant residues modulate conformational opening in SARS-CoV-2 spike protein.

PMID: 34615730 2021 Proc Natl Acad Sci U S A

Result: The B.1.1.7 also shows a P681H mutation close to the highly correlated N679 residue predicted from our model (SI Appendix.

CRISPR-Cas12a-Based Detection for the Major SARS-CoV-2 Variants of Concern.

PMID: 34787487 2021 Microbiology spectrum

Method: The SARS-CoV-2 target sequences include (i) the wild-type (WT) gene fragment of S protein (S; nucleotides [nt] 21,563 to 25,384; GenBank accession number MN908947); (ii) the mutant gene fragments of S protein, including mutations L5F, D80A, D215G, R246I, K417N, L452R/Q, Y453F, T478K, E484Q/K, N501Y, A570D, D614G, P681H, A701V, T716I,

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