Result: Intriguingly, one of the genomes (EPI_ISL_1567985) encoded S-L452R, S-N501Y, and S-G1219V associated with six other amino acid changes in the spike (A570D, D614G, P681H, T716I, S982A, I1221V), most interestingly comprising D614G and also P681H (Figure 4B).
Monitoring SARS-CoV-2 Populations in Wastewater by Amplicon Sequencing and Using the Novel Program SAM Refiner.
Result: Sequences from the S1S2 amplicon matched lineage B.1.1.7 with '1841G(D614G) 2042A(P681H) 2147T(T716I)', lineage P.1 with '1841G(D614G) 1963T(H655Y) 2063T(A688V)' or the B.1 lineage with only the now ubiquitous D614G variation (Supplementary 12).
Delta spike P681R mutation enhances SARS-CoV-2 fitness over Alpha variant.
Method: Individual point mutations for Alpha (NSP3: P153L, T183I, A890D, I1412T; NSP6: SGF106-108del; NSP12: P323L; Spike: HV69-70del, Y145del, N5
Result: It should be noted that the Alpha variant also has a spike mutation at amino acid position 681 (P681H), which may contribute to the increase in spike cleavage when compared with the wild-type USA/WA1-2020 virus; however, a recent study showed that mutation P681H alone did not enhance viral fitness or transmission.
Evolution, Mode of Transmission, and Mutational Landscape of Newly Emerging SARS-CoV-2 Variants.
Result: Having linked variants V18 and V22 together, as Ward's algorithm applied on Jaccard and Sorensen-Dice did, minimum edit distance equals three, as P812L non-defining substitution and deletion of defining substitutions: P681H, S982A mutually differ V18/V22.
Discussion: Given that P681H mutation enhances SARS-CoV-2 binding affinity towards the cell that results in increased infectivity, the absence of P681H mutation for V22, V9 and V23 may result in reduced infectivity.
Discussion: The absence of P681H mutation in this case can be associated to decreased SARS-CoV-2 binding affinity towards host cell, what is expected to result in reduced infectivity and mild up to moderate clinical presentation.
Discussion: Variant V11 is predicted to emerged from V4 upon delet
Crucial Mutations of Spike Protein on SARS-CoV-2 Evolved to Variant Strains Escaping Neutralization of Convalescent Plasmas and RBD-Specific Monoclonal Antibodies.
Introduction: In addition, several recent studies have focused on the neutralizing activity of vaccine-elicited humoral immunity against new circulating mutant lineages, including B.1.1.7 (United Kingdom, bearing mutations 69-70 del, 144 del, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H in the spike protein), B.1.429 (United States, bearing mutations S13I, W152C, L452R, and D614G in the spike protein), B.1.351(South Africa, bearing mutations D80A, D215G, PMID: 34488225
2021
Nature
Introduction: We previously showed that B.1.1.7 spike, bearing P681H, had significantly higher fusogenic potential than a D614G Wuhan-1 virus.
Impact of the Delta variant on vaccine efficacy and response strategies.
Introduction: P681R/P681H also exists in several variants under investigation in the United Kingdom, including A.23.1/E484K, B.1.1.7, and B1.318.
Emergence of SARS-CoV-2 Variant B.1.575.2, Containing the E484K Mutation in the Spike Protein, in Pamplona, Spain, May to June 2021.
PMID: 34495709
2021
Journal of clinical microbiology
Introduction: The B.1.575.1 sublineage was classified in the Phylogenetic Assignment of Named Global Outbreak (PANGO) lineage system as a Spanish sublineage of B.1.575 with spike mutations P681H, S494P, and T716I, and the B.1.575.2 sublineage, whose main characteristic is the presence of the E484K spike mutation, also originated in Spain.
Result: Among the common substitutions present in these lineages, four occurred in the spike protein (S494P, D614G, P681H, and T716I).
Dynamics prediction of emerging notable spike protein mutations in SARS-CoV-2 implies a need for updated vaccines.
Result: 4 and Tables 4 and 5 The summary of our superimposition results includes that the following we-named first group of variants; 20A/S.A262S, 20A/S.L452R, 20A/S.N501T, 20A/S.N501Y, 20A/S.P681H, 20A/S.P681R, 20A/S.V1176F, 20A/S.N439K, 20A/S.S98F, 20A/S.L5F, 20A/S.P272L, 20A/S.D1163Y, 20A/S.N439K, 20A/S.S98F, 20A/S.L5F, 20A/S.P272L, 20A/S.D1163Y and 20A/S.G1167V have
SARS_CoV_2 mutation literature information.
PMID: 
2021
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